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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Rat IL-2 Rabbit Recombinant Antibody, PBS Only

IL-2 Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

rat

Applications

FC (Intra)

Conjugate

Unconjugated

CloneNo.

240264C7

Cat no : 98046-1-PBS

Synonyms

Il2, interleukin 2, 240264C7

Validation Data Gallery

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  • Flow cytometry (FC) experiment of rat splenocytes cells using Anti-Rat IL-2 Rabbit Recombinant Antibody (98046-1-RR)

    FC experiment of rat splenocytes using 98046-1-RR (same clone as 98046-1-PBS)

    1x10^6 unstimulated or PMA, Ionomycin and Brefeldin A treated rat splenocytes were intracellularly stained with APC Anti-Rat CD4 (OX-35) and 0.25 ug Anti-Rat IL-2 Rabbit Recombinant Antibody (98046-1-RR, Clone: 240264C7) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98046-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of rat splenocytes cells using Anti-Rat IL-2 Rabbit Recombinant Antibody (98046-1-RR)

    FC experiment of rat splenocytes using 98046-1-RR (same clone as 98046-1-PBS)

    1x10^6 PMA, Ionomycin and Brefeldin A treated rat splenocytes were intracellularly stained with 0.25 ug Anti-Rat IL-2 Rabbit Recombinant Antibody (98046-1-RR, Clone: 240264C7) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98046-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 98046-1-RR

    Affinity and Kinetic Characterization of 98046-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98046-1-RR against Rat IL2 were performed. The affinity constant is 0.36 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 98046-1-PBS is IL-2 Fusion Protein expressed in E. coli.

Tested Reactivity rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name interleukin 2
Calculated molecular weight18 kDa
GenBank accession numberNM_053836
Gene symbol Il2
Gene ID (NCBI) 116562
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.