IL-22RA1 Polyclonal antibody

IL-22RA1 Polyclonal Antibody for WB, IF, ELISA, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 13462-1-AP

Synonyms

IL22RA1, IL22R, IL 22RA1, IL 22R alpha 1, IL 22 receptor subunit alpha 1


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Tested Applications

Positive WB detected inCOLO 320 cells, HEK-293 cells, HepG2 cells, HT-29 cells, mouse small intestine tissue, mouse heart tissue
Positive IF detected inhuman gut
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13462-1-AP targets IL-22RA1 in WB, IHC, IF, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IL-22RA1 fusion protein Ag4056 相同性解析による交差性が予測される生物種
Full Name interleukin 22 receptor, alpha 1
Calculated molecular weight 574 aa, 63 kDa
Observed molecular weight 63-68 kDa
GenBank accession numberBC029273
Gene symbol IL-22RA1
Gene ID (NCBI) 58985
RRIDAB_2248953
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Interleukin-22 (IL-22), a member of the IL-10 family of cytokines, is important for the modulation of tissue responses during inflammation. IL-22 receptor is a heterodimer of the IL-10RB and IL-22RA1. IL-22RA1 belongs to the class II cytokine receptor family. It is expressed in a limited number of tissues including skin, colon, liver, lung, pancreas and kidney. The expression is lack in immune cells such as monocytes, T cells, and NK cells (PMID: 15308104). IL-22RA1 can also form a receptor for IL-20 and IL-24 with IL20RB (PMID: 23793061).

Protocols

Product Specific Protocols
WB protocol for IL-22RA1 antibody 13462-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseFC

PLoS Biol

Loss of adenomatous polyposis coli function renders intestinal epithelial cells resistant to the cytokine IL-22.

Authors - Yu Chen
humanWB

Oxid Med Cell Longev

IL-22 Protects against Biliary Ischemia-Reperfusion Injury after Liver Transplantation via Activating STAT3 and Reducing Apoptosis and Oxidative Stress Levels In Vitro and In Vivo.

Authors - Yi Bai
humanWB

Clin Sci (Lond)

HMGB1-induced autophagy facilitates hepatic stellate cells activation: a new pathway in liver fibrosis.

Authors - Jing Li
humanWB

Apoptosis

Elevated IL-22 in psoriasis plays an anti-apoptotic role in keratinocytes through mediating Bcl-xL/Bax.

Authors - Bo Wang
humanIHC

Biochim Biophys Acta Mol Basis Dis

IL-22 ameliorated cardiomyocyte apoptosis in cardiac ischemia/reperfusion injury by blocking mitochondrial membrane potential decrease, inhibiting ROS and cytochrome C.

Authors - Yang Che
humanWB

Life Sci

The potential of Diosgenin in treating psoriasis: Studies from HaCaT keratinocytes and imiquimod-induced murine model.

Authors - Shidi Wu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Iram (Verified Customer) (06-13-2022)

Very good antibody for my western blotting

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: colon cancer cells
FH

Susmita (Verified Customer) (03-11-2022)

Excellent antibody

  • Applications: Western Blot
  • Primary Antibody Dilution: WB: 1: 2000
  • Cell Tissue Type: PC cell line