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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IL-28A Monoclonal antibody

IL-28A Monoclonal Antibody for IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

3B4A1

Cat no : 60270-1-Ig

Synonyms

Cytokine Zcyto20, IFN lambda 2, IFNL2, IL 28A, IL28A, IL-28A, Interferon lambda 2, Interleukin 28A, ZCYTO20

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using IL-28A Monoclonal antibody (60270-1-Ig)

    IHC staining of human tonsillitis using 60270-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60270-1-Ig (IL-28A antibody at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using IL-28A Monoclonal antibody (60270-1-Ig)

    IHC staining of human tonsillitis using 60270-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60270-1-Ig (IL-28A antibody at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human small intestine tissue using IL-28A Monoclonal antibody (60270-1-Ig)

    IHC staining of human small intestine using 60270-1-Ig

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 60270-1-Ig (IL-28A antibody at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human small intestine tissue using IL-28A Monoclonal antibody (60270-1-Ig)

    IHC staining of human small intestine using 60270-1-Ig

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 60270-1-Ig (IL-28A antibody at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using IL-28A Monoclonal antibody (60270-1-Ig)

    IF Staining of human tonsillitis using 60270-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using IL-28A antibody (60270-1-Ig, Clone: 3B4A1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using IL-28A Monoclonal antibody (60270-1-Ig)

    IF Staining of human tonsillitis using 60270-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using IL-28A antibody (60270-1-Ig, Clone: 3B4A1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive IHC detected inhuman tonsillitis tissue, human small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman tonsillitis tissue

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 60270-1-Ig is IL-28A Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Affinity
KD=1.77 x 109M
KOff=4.10 x 10-5M
KOn=2.31 x 104M
Immunogen IL-28A fusion protein Ag18525 相同性解析による交差性が予測される生物種
Full Name interleukin 28A (interferon, lambda 2)
Calculated molecular weight 200 aa, 22 kDa
GenBank accession numberBC113583
Gene symbol IL-28A
Gene ID (NCBI) 282616
RRIDAB_2881390
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IL28A, also named as IFNL2 and ZCYT020, is a cytokine with immunomodulatory activity. It up-regulates MHC class I antigen expression. IL28A is a ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IFNLR1. The ligand/receptor complex seems to signal through the Jak-STAT pathway. It plays a significant role in the antiviral immune defense in the intestinal epithelium.

Protocols

Product Specific Protocols
IHC protocol for IL-28A antibody 60270-1-IgDownload protocol
IF protocol for IL-28A antibody 60270-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols