IL2RA Recombinant antibody, PBS Only (Detector)

Sandwich ELISA standard curve of MP00837-3, Human IL2RA Recombinant Matched Antibody Pair - PBS only. 83896-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0408. 83896-6-PBS was HRP conjugated as the detection antibody. Range: 31.25-2000 pg/mL

Serum of eight individual healthy human donors was measured. The human IL2RA concentration of detected samples was determined to be 993.9 pg/mL with a range of 567.3 - 1,718.0 pg/mL.

Human peripheral blood mononuclear cells (5 x 10^6 cells/mL) were cultured in DMEM supplemented with 5% fetal bovine serum, 50 μM β-mercaptoethanol, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. The cells were cultured unstimulated or stimulated with 10 μg/mL PHA for 5 days. Aliquots of the cell culture supernates were removed and assayed for levels of human IL2RA.