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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using IL-2RG Polyclonal antibody (27377-1-AP)

    WB analysis of Jurkat using 27377-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 27377-1-AP (IL2RG antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using IL-2RG Polyclonal antibody (27377-1-AP)

    WB analysis of K-562 using 27377-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 27377-1-AP (IL2RG antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using IL-2RG Polyclonal antibody (27377-1-AP)

    WB analysis of Raji using 27377-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 27377-1-AP (IL2RG antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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IL-2RG Polyclonal antibody

IL-2RG Polyclonal Antibody for WB, ELISA
Cat No. 27377-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

IL2RG, IL-2 receptor subunit gamma, IL 2RG, IL 2R subunit gamma, IL 2 receptor subunit gamma

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, K-562 cells, Raji cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27377-1-AP targets IL-2RG in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IL-2RG fusion protein Ag26388 相同性解析による交差性が予測される生物種
Full Name interleukin 2 receptor, gamma (severe combined immunodeficiency)
Calculated molecular weight 369 aa, 42 kDa
Observed molecular weight 64-70 kDa
GenBank accession numberBC014972
Gene Symbol IL2RG
Gene ID (NCBI) 3561
RRIDAB_2880857
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP31785
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The interleukin-2 receptor (IL-2R) is a heterotrimeric receptor consisting of three subunits, alpha (IL-2RA), beta (IL-2RB), and gamma (IL-2RG). IL-2RG, also known as CD132, is an important signaling component of many interleukin receptors, including those of interleukin -2, -4, -7 and -21, and is thus referred to as the common gamma chain. Mutations in the gene of IL-2RG cause X-linked severe combined immunodeficiency (XSCID), as well as X-linked combined immunodeficiency (XCID), a less severe immunodeficiency disorder.

Protocols

Product Specific Protocols
WB protocol for IL-2RG antibody 27377-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols