IL-3 Polyclonal antibody
IL-3 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
Cat no : 17835-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Recombinant protein, Recombinant protein protein |
Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 1 publications below |
Product Information
The immunogen of 17835-1-AP is IL-3 Fusion Protein expressed in E. coli.
Tested Reactivity | human |
Cited Reactivity | pig |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | IL-3 fusion protein Ag11839 相同性解析による交差性が予測される生物種 |
Full Name | interleukin 3 (colony-stimulating factor, multiple) |
Calculated molecular weight | 152 aa, 17 kDa |
Observed molecular weight | 20 kDa |
GenBank accession number | BC066273 |
Gene symbol | IL-3 |
Gene ID (NCBI) | 3562 |
RRID | AB_10644127 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Interleukin-3 (IL-3) is a multilineage hematopoietic growth factor that promotes the proliferation, differentiation and survival of early multilineage hematopoietic progenitors. In particular, this cytokine plays a key role in stimulating the proliferation and survival of myeloid precursors. It is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. This cytokine has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders (PMID: 24103757;2698876;2809196)
Protocols
Product Specific Protocols | |
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WB protocol for IL-3 antibody 17835-1-AP | Download protocol |
IHC protocol for IL-3 antibody 17835-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
JTCVS Open Intramyocardial injection of hypoxia-conditioned extracellular vesicles modulates apoptotic signaling in chronically ischemic myocardium |