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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IL3RA/CD123 Recombinant antibody

IL3RA/CD123 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240042E5

Cat no : 83243-3-RR

Synonyms

IL3RA, IL 3R subunit alpha, IL 3R alpha, IL 3 receptor subunit alpha, hIL 3Ra

Validation Data Gallery

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  • Western Blot (WB) analysis of HL-60 cells using IL3RA/CD123 Recombinant antibody (83243-3-RR)

    WB analysis of HL-60 using 83243-3-RR

    HL-60 cells were subjected to SDS PAGE followed by western blot with 83243-3-RR (IL3RA/CD123 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83243-3-RR

    Affinity and Kinetic Characterization of 83243-3-RR

    Biolayer interferometry (BLl) kinetic assays of 82672-1-RR against Human IL3RA/CD123 were performed. The affinity constant is 4.88 nM.

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Tested Applications

Positive WB detected inHL-60 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83243-3-RR is IL3RA/CD123 Fusion Protein expressed in E. coli.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen IL3RA/CD123 fusion protein Ag4452 相同性解析による交差性が予測される生物種
Full Name interleukin 3 receptor, alpha (low affinity)
Calculated molecular weight 378 aa, 43 kDa
Observed molecular weight70 kDa
GenBank accession numberBC035407
Gene symbol CD123
Gene ID (NCBI) 3563
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Interleukin-3 receptor subunit alpha (IL3RA), also known as CD123, belongs to the cytokine receptor family. It is a glycoprotein containing an extracellular domain, involving a predicted Ig-like domain, two FnIII domains, a transmembrane domain and an intracellular domain (PMID: 24513123). IL3RA is expressed on hematopoietic progenitor cells, plasmacytoid dendritic cells, basophils, monocytes, some B cells, and in a variety of hematologic malignancies (PMID: 10527461; 34855461). IL-3 initially binds to IL3RA and subsequently recruits the beta chain (CD131) to form the high-affinity receptor, resulting in the activation of a series of signaling pathways including the JAK/STAT, Ras-MAPK, and phosphatidylinositol 3-kinase pathways (PMID: 29296749). IL-3R signaling regulates the proliferation, survival, and differentiation of hematopoietic cells.

Protocols

Product Specific Protocols
WB protocol for IL3RA/CD123 antibody 83243-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols