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Species Reactivity
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IL3RA/CD123 Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: human

Detection: human

Applications

Sandwich ELISA

Range

Sandwich ELISA: 62.5-4000 pg/mL

Cat No : MP00176-4

Print datasheet

Synonyms

Validation Data Gallery

  • Sandwich ELISA standard curve of MP00176-4, Human IL3RA Recombinant Matched Antibody Pair - PBS only. 83243-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag4452. 83243-4-PBS was HRP conjugated as the detection antibody. Range: 62.5-4000 pg/mL

    Sandwich ELISA standard curve of MP00176-4

    Sandwich ELISA standard curve of MP00176-4, Human IL3RA Recombinant Matched Antibody Pair - PBS only. 83243-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag4452. 83243-4-PBS was HRP conjugated as the detection antibody. Range: 62.5-4000 pg/mL

  • THP-1 and HL-60 cell lysates were measured. The human ILR3A concentration of detected samples was determined to be 76,268.8 pg/mL (based on a 4.0 mg/mL extract load) in THP-1 cell lysate and 39,279.2 pg/mL (based on a 4.3 mg/mL extract load) in HL-60 cell lysate.

    Sample test of MP00176-4

    THP-1 and HL-60 cell lysates were measured. The human ILR3A concentration of detected samples was determined to be 76,268.8 pg/mL (based on a 4.0 mg/mL extract load) in THP-1 cell lysate and 39,279.2 pg/mL (based on a 4.3 mg/mL extract load) in HL-60 cell lysate.

Tested Applications

Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

MP00176-4 targets IL3RA/CD123 in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.

Range: 62.5-4000 pg/mL (Sandwich ELISA);

Capture antibody: IL3RA/CD123 Recombinant antibody, PBS Only (Capture) 83243-3-PBS (240042E5). 100 μg. Concentration 1 mgl/ml.

Detection antibody: IL3RA Recombinant antibody, PBS Only (Detector) 83243-4-PBS (240042A4). 100 μg. Concentration 1 mgl/ml.

Alternative IL3RA/CD123 matched antibody pairs: MP00176-1, MP00176-2, MP00176-3

Matched pair protein standard: Ag4452

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 83243-3-PBS
Tested Reactivity human
Immunogen IL3RA/CD123 fusion protein Ag4452
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name interleukin 3 receptor, alpha (low affinity)
Synonyms IL3RA, IL 3R subunit alpha, IL 3R alpha, IL 3 receptor subunit alpha, hIL 3Ra
GenBank accession numberBC035407
Gene symbol CD123
UniprotP26951
Gene ID (NCBI) 3563
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purfication
Storage Buffer PBS only
Storage Conditions Store at -80°C.
Catalog Number 83243-4-PBS
Tested Reactivity human
Immunogen IL3RA fusion protein Ag4452
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name interleukin 3 receptor, alpha (low affinity)
Synonyms IL 3R subunit alpha, IL 3R alpha, IL 3 receptor subunit alpha, hIL 3Ra, CD123
GenBank accession numberBC035407
Gene symbol CD123
UniprotP26951
Gene ID (NCBI) 3563
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.