Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, Jurkat cells, mouse liver tissue |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IF | See 1 publications below |
Product Information
23048-1-AP targets IL-4I1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | IL-4I1 fusion protein Ag19136 相同性解析による交差性が予測される生物種 |
| Full Name | interleukin 4 induced 1 |
| Calculated molecular weight | 567 aa, 63 kDa |
| Observed molecular weight | 70 kDa |
| GenBank accession number | BC131625 |
| Gene Symbol | IL-4I1 |
| Gene ID (NCBI) | 259307 |
| RRID | AB_3085705 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96RQ9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Interleukin-4 Induced Gene 1 (IL4I1) is a secreted L-phenylalanine oxidase mainly produced by inflammatory antigen-presenting cells-in particular, macrophages present in T helper type 1 granulomas and in various types of tumors. In the last decade, it has been shown that IL4I1 is involved in the fine control of B- and T-cell adaptive immune responses. Preclinical models have revealed its role in cancer immune evasion. Recent clinical data highlight IL4I1 as a new potential prognostic marker in human melanoma. As a secreted enzyme, IL4I1 may represent an easily targetable molecule for cancer immunotherapy. IL4I1 protein has an estimated molecular weight of 75 kDa. (PMID: 31330829, PMID: 21469114)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for IL-4I1 antibody 23048-1-AP | Download protocol |
| IF protocol for IL-4I1 antibody 23048-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Commun Biol Single-cell dissection of multifocal bladder cancer reveals malignant and immune cells variation between primary and recurrent tumor lesions |