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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IL-4I1 Polyclonal antibody

IL-4I1 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 23048-1-AP

Synonyms

IL4I1, IL4 induced protein 1, hIL4I1, hFIG1, FIG1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IL-4I1 Polyclonal antibody (23048-1-AP)

    WB analysis using 23048-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23048-1-AP (IL-4I1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using IL-4I1 Polyclonal antibody (23048-1-AP)

    IF Staining of HeLa using 23048-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using IL-4I1 antibody (23048-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, mouse liver tissue
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

23048-1-AP targets IL-4I1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IL-4I1 fusion protein Ag19136 相同性解析による交差性が予測される生物種
Full Name interleukin 4 induced 1
Calculated molecular weight 567 aa, 63 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC131625
Gene symbol IL-4I1
Gene ID (NCBI) 259307
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Interleukin-4 Induced Gene 1 (IL4I1) is a secreted L-phenylalanine oxidase mainly produced by inflammatory antigen-presenting cells-in particular, macrophages present in T helper type 1 granulomas and in various types of tumors. In the last decade, it has been shown that IL4I1 is involved in the fine control of B- and T-cell adaptive immune responses. Preclinical models have revealed its role in cancer immune evasion. Recent clinical data highlight IL4I1 as a new potential prognostic marker in human melanoma. As a secreted enzyme, IL4I1 may represent an easily targetable molecule for cancer immunotherapy. IL4I1 protein has an estimated molecular weight of 75 kDa. (PMID: 31330829, PMID: 21469114)

Protocols

Product Specific Protocols
WB protocol for IL-4I1 antibody 23048-1-APDownload protocol
IF protocol for IL-4I1 antibody 23048-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols