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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IL-4R Polyclonal antibody

IL-4R Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28331-1-AP

Synonyms

CD124, IL 4 binding protein, IL 4 receptor subunit alpha, IL 4R alpha, IL 4R subunit alpha, IL 4RA, IL4 BP, IL4R, IL-4R, IL4RA, interleukin 4 receptor, sIL4Ralpha/prot, Soluble IL 4R alpha

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using IL-4R Polyclonal antibody (28331-1-AP)

    WB analysis using 28331-1-AP

    Daudi cells were subjected to SDS PAGE followed by western blot with 28331-1-AP (IL4R antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using IL-4R Polyclonal antibody (28331-1-AP)

    IHC staining of human tonsillitis using 28331-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 28331-1-AP (IL4R antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using IL-4R Polyclonal antibody (28331-1-AP)

    IHC staining of human tonsillitis using 28331-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 28331-1-AP (IL4R antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inDaudi cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

28331-1-AP targets IL-4R in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IL-4R fusion protein Ag28766 相同性解析による交差性が予測される生物種
Full Name interleukin 4 receptor
Calculated molecular weight 90 kDa
Observed molecular weight 120-140 kDa
GenBank accession numberNM_000418
Gene symbol IL-4R
Gene ID (NCBI) 3566
RRIDAB_2881115
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for IL-4R antibody 28331-1-APDownload protocol
IHC protocol for IL-4R antibody 28331-1-APDownload protocol
FC protocol for IL-4R antibody 28331-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biomedicines

Exosomes from the Uterine Cavity Mediate Immune Dysregulation via Inhibiting the JNK Signal Pathway in Endometriosis

Authors - Ying Jiang
View Article
humanWB

Cancer Sci

CD44v5 domain regulates crosstalk between TNBC cells and tumor-associated macrophages by enhancing the IL-4R/STAT3 axis

Authors - Yanhua Dai
View Article