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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IL-5 Polyclonal antibody

IL-5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26677-1-AP

Synonyms

EDF, IL 5, IL5, IL-5, Interleukin 5, T cell replacing factor, TRF

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of Recombinant protein using IL-5 Polyclonal antibody (26677-1-AP)

    WB analysis of Recombinant protein using 26677-1-AP

    Recombinant protein were subjected to SDS PAGE followed by western blot with 26677-1-AP (IL5 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using IL-5 Polyclonal antibody (26677-1-AP)

    IHC staining of human breast cancer using 26677-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26677-1-AP (IL5 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using IL-5 Polyclonal antibody (26677-1-AP)

    IHC staining of human breast cancer using 26677-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26677-1-AP (IL5 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inRecombinant protein
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 26677-1-AP is IL-5 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IL-5 fusion protein Ag24583 相同性解析による交差性が予測される生物種
Full Name interleukin 5 (colony-stimulating factor, eosinophil)
Calculated molecular weight 134 aa, 15 kDa
GenBank accession numberBC066282
Gene symbol IL5
Gene ID (NCBI) 3567
RRIDAB_2880598
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for IL-5 antibody 26677-1-APDownload protocol
IHC protocol for IL-5 antibody 26677-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Laryngoscope

Eosinophilic Sialodochitis: A Type of Chronic Obstructive Sialadenitis Related to Allergy.

Authors - Wen-Xuan Zhu
View Article
humanIHC

Laryngoscope

Primary Hyper-IgE-Related Salivary Gland Disease: A New Disease Entity.

Authors - Wen-Xuan Zhu
View Article
humanWB

J Immunol Res

Respiratory Syncytial Virus Nonstructural Protein 1 Promotes 5-Lipoxygenase via miR-19a-3p.

Authors - Mei Zheng
View Article