ILK Polyclonal antibody

ILK Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12955-1-AP

Synonyms

ILK, ILK 1, ILK 2, ILK1, ILK2, P59, p59ILK


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Tested Applications

Positive WB detected inmouse brain tissue, mouse kidney tissue
Positive IP detected inmouse kidney tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12955-1-AP targets ILK in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ILK fusion protein Ag3570 相同性解析による交差性が予測される生物種
Full Name integrin-linked kinase
Calculated molecular weight 451 aa, 59 kDa
Observed molecular weight 51-59 kDa
GenBank accession numberBC001554
Gene symbol ILK
Gene ID (NCBI) 3611
RRIDAB_2127053
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Integrin-linked kinase (ILK) is an ankyrin-repeat containing serine-threonine protein kinase capable of interacting with the cytoplasmic domains of integrin β1, β2, and β3 subunits and whose kinase activity is modulated by cell-extracellular matrix interactions (PMID: 9539744). This protein belongs to the protein kinase superfamily. It can be autophosphorylated on serine residues.

Protocols

Product Specific Protocols
WB protocol for ILK antibody 12955-1-APDownload protocol
IHC protocol for ILK antibody 12955-1-APDownload protocol
IP protocol for ILK antibody 12955-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
pigWB,IP

Sci Total Environ

Protein lysine acetylation played an important role in NH3-induced AEC2 damage and pulmonary fibrosis in piglets

Authors - Xiaotong Wang
humanIP

FASEB J

Coiled-coil domain containing 25 (CCDC25) regulates cell proliferation, migration, and invasion in clear cell renal cell carcinoma by targeting the ILK-NF-κB signaling pathway

Authors - Zhenzhen Qian
humanWB

J Cancer

GTW inhibits the Epithelial to Mesenchymal Transition of Epithelial Ovarian Cancer via ILK/AKT/GSK3β/Slug Signalling Pathway.

Authors - Ying Feng
humanWB

Int Immunopharmacol

BML-111 inhibits EMT, migration and metastasis of TAMs-stimulated triple-negative breast cancer cells via ILK pathway.

Authors - Lan Lin
humanIHC

BMC Gastroenterol

Identification and validation of EMT-immune-related prognostic biomarkers CDKN2A, CMTM8 and ILK in colon cancer.

Authors - Ning Kang
mouseWB,IF

PLoS One

Cleavage of periostin by MMP9 protects mice from kidney cystic disease

Authors - Nabila Djaziri