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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ILK Polyclonal antibody, PBS Only

ILK Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 24926-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse heart tissue using ILK Polyclonal antibody (24926-1-AP)

    WB analysis of mouse heart using 24926-1-AP (same clone as 24926-1-PBS)

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 24926-1-AP (ILK Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24926-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using ILK Polyclonal antibody (24926-1-AP)

    WB analysis of MCF-7 using 24926-1-AP (same clone as 24926-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 24926-1-AP (ILK Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24926-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using ILK Polyclonal antibody (24926-1-AP)

    IHC staining of human pancreas cancer using 24926-1-AP (same clone as 24926-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 24926-1-AP (ILK Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24926-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using ILK Polyclonal antibody (24926-1-AP)

    IHC staining of human pancreas cancer using 24926-1-AP (same clone as 24926-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 24926-1-AP (ILK Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24926-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using ILK Polyclonal antibody (24926-1-AP)

    IF Staining of MCF-7 using 24926-1-AP (same clone as 24926-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 24926-1-AP (ILK antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 24926-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

24926-1-PBS targets ILK in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ILK fusion protein Ag21494 相同性解析による交差性が予測される生物種
Full Name integrin-linked kinase
Calculated molecular weight 451 aa, 59 kDa
Observed molecular weight 51 kDa
GenBank accession numberBC001554
Gene symbol ILK
Gene ID (NCBI) 3611
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

ILK(Integrin-linked protein kinase) is also named as p59, PAT-4, DKFZp686F1765, ILK-1, ILK-2, ILK1, ILK2, p59ILK and belongs to the TKL Ser/Thr protein kinase family. This protein localizes to multiple sites, including the cytoplasm, and imports into the nucleus through sequences in its N-terminus, via active transport mechanisms that involve nuclear pore complexes(PMID: 18635968). ILK performs crucial roles in the control of intestinal cell and crypt-villus axis homeostasis-especially with regard to basement membrane fibronectin deposition-as well as cell proliferation, spreading, and migration(PMID:19885839).