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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ILK Monoclonal antibody, PBS Only

ILK Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E10E4

Cat no : 67724-1-PBS

Synonyms

ILK 2, ILK 1, EC:2.7.11.1, Beta-integrin-linked kinase, 59 kDa serine/threonine-protein kinase

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using ILK Monoclonal antibody (67724-1-Ig)

    WB analysis using 67724-1-Ig (same clone as 67724-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67724-1-Ig (ILK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67724-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human peripheral blood platelets using ILK Monoclonal antibody (67724-1-Ig)

    WB analysis of human peripheral blood platelets using 67724-1-Ig (same clone as 67724-1-PBS)

    human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 67724-1-Ig (ILK antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67724-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human placenta tissue using ILK Monoclonal antibody (67724-1-Ig)

    WB analysis of human placenta using 67724-1-Ig (same clone as 67724-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 67724-1-Ig (ILK antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67724-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig colon tissue using ILK Monoclonal antibody (67724-1-Ig)

    WB analysis of pig colon using 67724-1-Ig (same clone as 67724-1-PBS)

    pig colon tissue were subjected to SDS PAGE followed by western blot with 67724-1-Ig (ILK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67724-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse colon tissue using ILK Monoclonal antibody (67724-1-Ig)

    WB analysis of mouse colon using 67724-1-Ig (same clone as 67724-1-PBS)

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 67724-1-Ig (ILK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67724-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat colon tissue using ILK Monoclonal antibody (67724-1-Ig)

    WB analysis of rat colon using 67724-1-Ig (same clone as 67724-1-PBS)

    rat colon tissue were subjected to SDS PAGE followed by western blot with 67724-1-Ig (ILK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67724-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using ILK Monoclonal antibody (67724-1-Ig)

    IHC staining of human skin cancer using 67724-1-Ig (same clone as 67724-1-PBS)

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 67724-1-Ig (ILK antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67724-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67724-1-PBS targets ILK in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ILK fusion protein Ag21289 相同性解析による交差性が予測される生物種
Full Name integrin-linked kinase
Calculated molecular weight 451 aa, 59 kDa
Observed molecular weight51 kDa
GenBank accession numberBC001554
Gene symbol ILK
Gene ID (NCBI) 3611
RRIDAB_2882910
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

ILK(Integrin-linked protein kinase) is also named as p59, PAT-4, DKFZp686F1765, ILK-1, ILK-2, ILK1, ILK2, p59ILK and belongs to the TKL Ser/Thr protein kinase family. This protein localizes to multiple sites, including the cytoplasm, and imports into the nucleus through sequences in its N-terminus, via active transport mechanisms that involve nuclear pore complexes(PMID: 18635968). ILK performs crucial roles in the control of intestinal cell and crypt-villus axis homeostasis-especially with regard to basement membrane fibronectin deposition-as well as cell proliferation, spreading, and migration(PMID:19885839).