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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IMMT Monoclonal antibody (68226-1-Ig)

    WB analysis using 68226-1-Ig (same clone as 68226-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68226-1-Ig (IMMT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68226-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using IMMT Monoclonal antibody (68226-1-Ig)

    WB analysis of HeLa using 68226-1-Ig (same clone as 68226-1-PBS)

    WB result of IMMT antibody (68226-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IMMT transfected HeLa cells. This data was developed using the same antibody clone with 68226-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using IMMT Monoclonal antibody (68226-1-Ig)

    IHC staining of mouse heart using 68226-1-Ig (same clone as 68226-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 68226-1-Ig (IMMT antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68226-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using IMMT Monoclonal antibody (68226-1-Ig)

    IHC staining of mouse heart using 68226-1-Ig (same clone as 68226-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 68226-1-Ig (IMMT antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68226-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using IMMT Monoclonal antibody (68226-1-Ig)

    IF Staining of HeLa using 68226-1-Ig (same clone as 68226-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using IMMT antibody (68226-1-Ig, Clone: 2D2E8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68226-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using IMMT Monoclonal antibody (68226-1-Ig)

    IF Staining of HeLa using 68226-1-Ig (same clone as 68226-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using IMMT antibody (68226-1-Ig, Clone: 2D2E8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68226-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HEK-293 cells using IMMT Monoclonal antibody (68226-1-Ig)

    FC experiment of HEK-293 using 68226-1-Ig (same clone as 68226-1-PBS)

    1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human IMMT (68226-1-Ig, Clone:2D2E8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68226-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50515-1

    Cytometric bead array standard curve of MP50515-1

    Cytometric bead array standard curve of MP50515-1, IMMT Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68226-1-PBS. Detection antibody: 68226-2-PBS. Standard:Ag0102. Range: 0.098-100 ng/mL.

  • Cytometric bead array standard curve of MP50515-2

    Cytometric bead array standard curve of MP50515-2

    Cytometric bead array standard curve of MP50515-2, IMMT Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68226-3-PBS. Detection antibody: 68226-1-PBS. Standard:Ag0102. Range: 0.098-100 ng/mL.

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  • Featured Product
  • KD/KO Validated

IMMT Monoclonal antibody, PBS Only (Capture/Detector)

IMMT Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 68226-1-PBS
Clone No.2D2E8

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Synonyms

MINOS2, MICOS complex subunit MIC60, MIC60, HMP, Cell proliferation-inducing gene 4/52 protein

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68226-1-PBS targets IMMT as part of a matched antibody pair:

MP50515-1: 68226-1-PBS capture and 68226-2-PBS detection (validated in Cytometric bead array)

MP50515-2: 68226-3-PBS capture and 68226-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen IMMT fusion protein Ag0102 相同性解析による交差性が予測される生物種
Full Name inner membrane protein, mitochondrial (mitofilin)
Calculated molecular weight 90 kDa
Observed molecular weight90 kDa
GenBank accession numberBC002412
Gene Symbol IMMT
Gene ID (NCBI) 10989
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ16891
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

IMMT (also known as mitofilin) is an inner mitochondrial membrane protein that is preferentially expressed in heart tissue and is commonly used as the marker for mitochondria. Mitofilin is known to be a critical organizer of mitochondrial cristae morphology and is indispensable for normal mitochondrial function. Three isoforms of mitofilin exist due to the alternative splicing. All three proteins of 87kD, 89kD and 80kD can be detected in immunoblot analysis with this antibody.