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  • Featured Product
  • KD/KO Validated

IMMT Monoclonal antibody, PBS Only

IMMT Monoclonal Antibody for WB, IF, FC, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Rat, Mouse

Applications

WB, IF, FC, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2D2E8

Cat no : 68226-1-PBS

Synonyms

DKFZp779P1653, HMP, IMMT, Mitofilin, P87, P87/89, P89, PIG4, PIG52

Validation Data Gallery

Applications:
  • WB analysis using 68226-1-Ig (same clone as 68226-1-PBS)

    WB analysis using 68226-1-Ig (same clone as 68226-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68226-1-Ig (IMMT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68226-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 68226-1-Ig (same clone as 68226-1-PBS)

    WB analysis of HeLa using 68226-1-Ig (same clone as 68226-1-PBS)

    WB result of IMMT antibody (68226-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IMMT transfected HeLa cells. This data was developed using the same antibody clone with 68226-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 68226-1-Ig (same clone as 68226-1-PBS)

    IHC staining of mouse heart using 68226-1-Ig (same clone as 68226-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 68226-1-Ig (IMMT antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68226-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with IMMT antibody included
  • IHC staining of mouse heart using 68226-1-Ig (same clone as 68226-1-PBS)

    IHC staining of mouse heart using 68226-1-Ig (same clone as 68226-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 68226-1-Ig (IMMT antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68226-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with IMMT antibody included
  • IF Staining of HeLa using 68226-1-Ig (same clone as 68226-1-PBS)

    IF Staining of HeLa using 68226-1-Ig (same clone as 68226-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using IMMT antibody (68226-1-Ig, Clone: 2D2E8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68226-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 68226-1-Ig (same clone as 68226-1-PBS)

    IF Staining of HeLa using 68226-1-Ig (same clone as 68226-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using IMMT antibody (68226-1-Ig, Clone: 2D2E8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68226-1-PBS in a different storage buffer formulation.

  • FC experiment of HEK-293 using 68226-1-Ig (same clone as 68226-1-PBS)

    FC experiment of HEK-293 using 68226-1-Ig (same clone as 68226-1-PBS)

    1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human IMMT (68226-1-Ig, Clone:2D2E8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68226-1-PBS in a different storage buffer formulation.

Tested Applications

Planning an IHC experiment? We recommend our IHCeasy IMMT Ready-To-Use IHC Kit. IMMT primary antibody included.

Recommended dilution

ApplicationDilution
Sample-dependent, check data in validation data gallery

Product Information

68226-1-PBS targets IMMT in WB, IF, FC, IHC, Indirect ELISA applications and shows reactivity with Human, Rat, Mouse samples.

Tested Reactivity Human, Rat, Mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen IMMT fusion protein Ag0102 相同性解析による交差性が予測される生物種
Full Name inner membrane protein, mitochondrial (mitofilin)
Calculated molecular weight 90 kDa
Observed molecular weight90 kDa
GenBank accession numberBC002412
Gene symbol IMMT
Gene ID (NCBI) 10989
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

IMMT (also known as mitofilin) is an inner mitochondrial membrane protein that is preferentially expressed in heart tissue and is commonly used as the marker for mitochondria. Mitofilin is known to be a critical organizer of mitochondrial cristae morphology and is indispensable for normal mitochondrial function. Three isoforms of mitofilin exist due to the alternative splicing. All three proteins of 87kD, 89kD and 80kD can be detected in immunoblot analysis with this antibody.