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  • Featured Product
  • KD/KO Validated

IMP3 Polyclonal antibody

IMP3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 12750-1-AP

Synonyms

MRPS4, IMP 3, C15orf12, BRMS2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IMP3 Polyclonal antibody (12750-1-AP)

    WB analysis using 12750-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12750-1-AP (IMP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using IMP3 Polyclonal antibody (12750-1-AP)

    WB analysis of HeLa using 12750-1-AP

    WB result of IMP3 antibody (12750-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IMP3 transfected HeLa cells.

  • Immunoprecipitation (IP) experiment of HeLa cells using IMP3 Polyclonal antibody (12750-1-AP)

    IP experiment of HeLa using 12750-1-AP

    IP result of anti-IMP3 (IP:12750-1-AP, 3ug; Detection:12750-1-AP 1:500) with HeLa cells lysate 1200ug.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using IMP3 Polyclonal antibody (12750-1-AP)

    IHC staining of human ovary cancer using 12750-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 12750-1-AP (IMP3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using IMP3 Polyclonal antibody (12750-1-AP)

    IF Staining of HepG2 using 12750-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using IMP3 antibody (12750-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12750-1-AP targets IMP3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IMP3 fusion protein Ag3477 相同性解析による交差性が予測される生物種
Full Name IMP3, U3 small nucleolar ribonucleoprotein, homolog (yeast)
Calculated molecular weight 184 aa, 22 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC020557
Gene symbol IMP3
Gene ID (NCBI) 55272
RRIDAB_2122784
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IMP U3 small nucleolar ribonucleoprotein 3 (IMP3, BRMS2), is a component of U3 small nucleolar ribonucleoprotein complex (U3 snoRNP), has been identified as a participant in pre-rRNA processing for nearly twenty years.

Protocols

Product Specific Protocols
WB protocol for IMP3 antibody 12750-1-APDownload protocol
IHC protocol for IMP3 antibody 12750-1-APDownload protocol
IF protocol for IMP3 antibody 12750-1-APDownload protocol
IP protocol for IMP3 antibody 12750-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IHC

Oncotarget

IMP2/IGF2BP2 expression, but not IMP1 and IMP3, predicts poor outcome in patients and high tumor growth rate in xenograft models of gallbladder cancer.

Authors - Sonja M Kessler
View Article