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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IMP4 Polyclonal antibody

IMP4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16205-1-AP

Synonyms

IMP 4, BXDC4, Brix domain-containing protein 4

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using IMP4 Polyclonal antibody (16205-1-AP)

    WB analysis of HeLa using 16205-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16205-1-AP (IMP4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using IMP4 Polyclonal antibody (16205-1-AP)

    WB analysis of HepG2 using 16205-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16205-1-AP (IMP4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using IMP4 Polyclonal antibody (16205-1-AP)

    IHC staining of human ovary cancer using 16205-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 16205-1-AP (IMP4 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHeLa cells, HepG2 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

16205-1-AP targets IMP4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IMP4 fusion protein Ag9175 相同性解析による交差性が予測される生物種
Full Name IMP4, U3 small nucleolar ribonucleoprotein, homolog (yeast)
Calculated molecular weight 291 aa, 34 kDa
Observed molecular weight 34 kDa
GenBank accession numberBC010042
Gene symbol IMP4
Gene ID (NCBI) 92856
RRIDAB_2239858
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for IMP4 antibody 16205-1-APDownload protocol
IHC protocol for IMP4 antibody 16205-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

iScience

Multi-omics approach reveals posttranscriptionally regulated genes are essential for human pluripotent stem cells.

Authors - Mio Iwasaki
View Article