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KD/KO Validated

IMPDH2 Polyclonal antibody

Name: IMPDH2 Polyclonal antibody
Brand: Proteintech
SKU: 12948-1-AP

IMPDH2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, RIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 12948-1-AP

Synonyms

IMPDH II, IMPDH 2, IMPD2, IMPD 2, IMP dehydrogenase II

Validation Data Gallery

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AllWBIPIHCIF/ICC

WB analysis of A549 using 12948-1-AP
WB result of IMPDH2 antibody (12948-1-AP; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IMPDH2 transfected A549 cells.
WB analysis using 12948-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12948-1-AP (IMPDH2 antibody) at dilution of 1:18000 incubated at room temperature for 1.5 hours.
WB analysis of rat heart using 12948-1-AP
rat heart tissue were subjected to SDS PAGE followed by western blot with 12948-1-AP (IMPDH2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 12948-1-AP
IP result of anti-IMPDH2 (IP:12948-1-AP, 4ug; Detection:12948-1-AP 1:20000) with HeLa cells lysate 1320 ug.
IHC staining of human lung cancer using 12948-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12948-1-AP (IMPDH2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 12948-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12948-1-AP (IMPDH2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 12948-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using IMPDH2 antibody (12948-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Cat No. 12948-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, rat heart tissue, A549 cells, MCF-7 cells, K-562 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human lung cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 5 publications below
WB	See 28 publications below
IHC	See 5 publications below
IF	See 33 publications below
IP	See 1 publications below
ELISA	See 1 publications below
RIP	See 1 publications below

Product Information

12948-1-AP targets IMPDH2 in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, zebrafish
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	IMPDH2 fusion protein Ag3632 相同性解析による交差性が予測される生物種
Full Name	IMP (inosine monophosphate) dehydrogenase 2
Calculated molecular weight	514 aa, 56 kDa
Observed molecular weight	56 kDa
GenBank accession number	BC012840
Gene symbol	IMPDH2
Gene ID (NCBI)	3615
RRID	AB_2127351
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

IMPDH2, also named as IMPD2, belongs to the IMPDH/GMPR family. It is a rate limiting enzyme in the de novo synthesis of guanine nucleotides and therefore is involved in the regulation of cell growth. It may also have a role in the development of malignancy and the growth progression of some tumors. IMPDH2 is a key enzyme in the purine nucleotide biosynthetic pathway and constitutes a pivotal biological target for immunosuppressant and antiviral drugs. (PMID:21181270) IMPDH2 is highly enriched in RR(filamentous rods and rings). This antibody may have cross-reaction with IMPDH1 due to the high homology.

Protocols

Product Specific Protocols
WB protocol for IMPDH2 antibody 12948-1-AP	Download protocol
IHC protocol for IMPDH2 antibody 12948-1-AP	Download protocol
IF protocol for IMPDH2 antibody 12948-1-AP	Download protocol
IP protocol for IMPDH2 antibody 12948-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Mol Cell ERK2 Phosphorylates PFAS to Mediate Posttranslational Control of De Novo Purine Synthesis. Authors - Eunus S Ali View Article
mouse	IF	J Clin Invest Hirschsprung-like disease is exacerbated by reduced de novo GMP synthesis. Authors - Jonathan I Lake View Article
human	WB	Mol Ther IMPDH1/YB-1 Positive Feedback Loop Assembles Cytoophidia and Represents a Therapeutic Target in Metastatic Tumors. Authors - Hailong Ruan KD Validated View Article
human	IF	Autoimmun Rev Anti-rods/rings autoantibodies in a patient with pancreatic injury. Authors - Shingo Katayama View Article
human	RIP	Cancer Lett Circular RNA circPFKP promotes cell proliferation by activating IMPDH2 in prostate cancer. Authors - Shiyu Wang View Article
human	WB	Elife SARS-CoV-2 Nsp14 mediates the effects of viral infection on the host cell transcriptome. Authors - Michela Zaffagni View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IMPDH2 Polyclonal antibody

IMPDH2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis of A549 using 12948-1-AP

WB analysis using 12948-1-AP

WB analysis of rat heart using 12948-1-AP

IP experiment of HeLa using 12948-1-AP

IHC staining of human lung cancer using 12948-1-AP

IHC staining of human lung cancer using 12948-1-AP

IF Staining of HeLa using 12948-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

ERK2 Phosphorylates PFAS to Mediate Posttranslational Control of De Novo Purine Synthesis.

Hirschsprung-like disease is exacerbated by reduced de novo GMP synthesis.

IMPDH1/YB-1 Positive Feedback Loop Assembles Cytoophidia and Represents a Therapeutic Target in Metastatic Tumors.

Anti-rods/rings autoantibodies in a patient with pancreatic injury.

Circular RNA circPFKP promotes cell proliferation by activating IMPDH2 in prostate cancer.

SARS-CoV-2 Nsp14 mediates the effects of viral infection on the host cell transcriptome.