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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IMUP Recombinant antibody, PBS Only

IMUP Recombinant Antibody for IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241220B4

Cat no : 84148-6-PBS

Synonyms

Immortalization up-regulated protein, Hepatocyte growth factor activator inhibitor type 2-related small protein, HAI-2-related small protein, H2RSP, C19orf33

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human skin cancer tissue using IMUP Recombinant antibody (84148-6-RR)

    IHC staining of human skin cancer using 84148-6-RR (same clone as 84148-6-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 84148-6-RR (IMUP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84148-6-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using IMUP Recombinant antibody (84148-6-RR)

    IHC staining of human skin cancer using 84148-6-RR (same clone as 84148-6-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 84148-6-RR (IMUP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84148-6-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using IMUP Recombinant antibody (84148-6-RR)

    IF Staining of A431 using 84148-6-RR (same clone as 84148-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using C19orf33 antibody (84148-6-RR, Clone: 241220B4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84148-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84148-6-RR

    Affinity and Kinetic Characterization of 84148-6-RR

    Biolayer interferometry (BLl) kinetic assays of 84148-6-RR against Human IMUP were performed. The affinity constant is 10.3 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 84148-6-PBS is IMUP Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen IMUP fusion protein Ag22218 相同性解析による交差性が予測される生物種
Full Name chromosome 19 open reading frame 33
Calculated molecular weight 106 aa, 11 kDa
GenBank accession numberBC060319
Gene symbol IMUP
Gene ID (NCBI) 64073
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Immortalization up-regulated protein (IMUP), also known as C19orf33 or H2RSP, was first identified in SV40-immortalized fibroblasts and includes two isoforms, IMUP-1 and IMUP-2 (PMID: 14981917; 35142956). It is found primarily in the nucleus (PMID: 11606055). IMUP is reportedly upregulated in many cancer cells and tissues and its overexpression is associated with tumorigenicity and cell-cycle acceleration (PMID: 16962562; 35142956; 22886722). The apparent molecular determined by SDS-PAGE might be due to post-translational modifications or anomalous migration behavior (PMID: 11080599).