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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

INF2 Monoclonal antibody, PBS Only

INF2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4A11A7

Cat no : 66910-1-PBS

Synonyms

C14orf151, C14orf173, DKFZp762A0214, FLJ22056, HBEBP2 binding protein C, INF2, Inverted formin 2, pp9484

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using INF2 Monoclonal antibody (66910-1-Ig)

    WB analysis using 66910-1-Ig (same clone as 66910-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66910-1-Ig (INF2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66910-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using INF2 Monoclonal antibody (66910-1-Ig)

    IHC staining of human kidney using 66910-1-Ig (same clone as 66910-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66910-1-Ig (INF2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66910-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using INF2 Monoclonal antibody (66910-1-Ig)

    IHC staining of human kidney using 66910-1-Ig (same clone as 66910-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66910-1-Ig (INF2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66910-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using INF2 Monoclonal antibody (66910-1-Ig)

    IF Staining of HepG2 using 66910-1-Ig (same clone as 66910-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using INF2 antibody (66910-1-Ig, Clone: 4A11A7 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66910-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66910-1-PBS targets INF2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen INF2 fusion protein Ag17043 相同性解析による交差性が予測される生物種
Full Name inverted formin, FH2 and WH2 domain containing
Calculated molecular weight 1249 aa, 136 kDa
Observed molecular weight 150-170 kDa
GenBank accession numberBC008756
Gene symbol INF2
Gene ID (NCBI) 64423
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

INF2 is a member of the formin family of actin-regulating proteins. INF2 is peripherally bound to the cytoplasmic face of the endoplasmic reticulum (ER). Mutations in the formin gene INF2 cause focal segmental glomerulosclerosis. The predicted MW of INF2 is around 136 kDa, while higher molecular mass from 140-200 kDa has been reported in literature (26383224,20493814,19366733).