ING1-specific Polyclonal antibody
ING1-specific Polyclonal Antibody for WB, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
WB, IP, ELISA
Conjugate
Unconjugated
Cat no : 16185-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | human heart tissue |
Positive IP detected in | mouse heart tissue |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
16185-1-AP targets ING1-specific in WB, IP, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Cited Reactivity | rat' |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
Full Name | inhibitor of growth family, member 1 |
Calculated molecular weight | 47 kDa |
Observed molecular weight | 30 kDa |
GenBank accession number | NM_198218 |
Gene symbol | ING1 |
Gene ID (NCBI) | 3621 |
RRID | AB_2233908 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Members of inhibitor of growth (ING) family function in inhibiting cell growth and inducing apoptosis. They are sequence homologous proteins. ING1 is the first identified one, interacting with TP53 and is a component of the p53 signaling pathway. Multiple alternatively spliced transcript variants encoding distinct isoforms of ING1 have been reported. This antibody recognizes all 24/33/47kd isoforms of ING1, but will not bind ING2, 3 or 4.
Protocols
Product Specific Protocols | |
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WB protocol for ING1-specific antibody 16185-1-AP | Download protocol |
IP protocol for ING1-specific antibody 16185-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |