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Validation Data Gallery

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  • Western Blot (WB) analysis of human heart tissue using ING1-specific Polyclonal antibody (16185-1-AP)

    WB analysis of human heart using 16185-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 16185-1-AP (ING1-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using ING1-specific Polyclonal antibody (16185-1-AP)

    WB analysis of human heart using 16185-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 16185-1-AP (ING1-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse heart tissue using ING1-specific Polyclonal antibody (16185-1-AP)

    IP experiment of mouse heart using 16185-1-AP

    IP result of anti-ING1-specific (IP:16185-1-AP, 2ug; Detection:16185-1-AP 1:300) with mouse heart tissue lysate 4000ug.

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ING1-specific Polyclonal antibody

ING1-specific Polyclonal Antibody for WB, IP, ELISA
Cat No. 16185-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, ELISA

Synonyms

ING1, p24ING1c, p33, p33ING1, p33ING1b, p47, p47ING1a

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman heart tissue
Positive IP detected inmouse heart tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16185-1-AP targets ING1-specific in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity rat'
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name inhibitor of growth family, member 1
Calculated molecular weight 47 kDa
Observed molecular weight 30 kDa
GenBank accession numberNM_198218
Gene Symbol ING1
Gene ID (NCBI) 3621
RRIDAB_2233908
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UK53
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Members of inhibitor of growth (ING) family function in inhibiting cell growth and inducing apoptosis. They are sequence homologous proteins. ING1 is the first identified one, interacting with TP53 and is a component of the p53 signaling pathway. Multiple alternatively spliced transcript variants encoding distinct isoforms of ING1 have been reported. This antibody recognizes all 24/33/47kd isoforms of ING1, but will not bind ING2, 3 or 4.

Protocols

Product Specific Protocols
WB protocol for ING1-specific antibody 16185-1-APDownload protocol
IP protocol for ING1-specific antibody 16185-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols