Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, human liver tissue |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human colon cancer tissue, human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 7 publications below |
| IHC | See 3 publications below |
| IF | See 1 publications below |
| IP | See 1 publications below |
Product Information
11560-1-AP targets ING2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ING2 fusion protein Ag2121 相同性解析による交差性が予測される生物種 |
| Full Name | inhibitor of growth family, member 2 |
| Calculated molecular weight | 280 aa, 33 kDa |
| Observed molecular weight | 33 kDa |
| GenBank accession number | BC030128 |
| Gene Symbol | ING2 |
| Gene ID (NCBI) | 3622 |
| RRID | AB_2127771 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H160 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ING2, also named as ING1L, belongs to the ING family and contains a PHD-type zinc finger. It is induced by the DNA-damaging agent neocarzinostatin. As a candidate tumor suppressor, ING2 seems to be involved in p53/TP53 activation and p53/TP53-dependent apoptotic pathways, probably by enhancing acetylation of p53/TP53. It is a component of a mSin3A-like corepressor complex, which is probably involved in deacetylation of nucleosomal histones. Recent finding reported that ING2 regulates muscle differentiation by regulating chormatin remodeling program. Catalog#11560-1-AP is a rabbit polyclonal antibody raised against full length ING2 of human origin.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ING2 antibody 11560-1-AP | Download protocol |
| IHC protocol for ING2 antibody 11560-1-AP | Download protocol |
| IF protocol for ING2 antibody 11560-1-AP | Download protocol |
| IP protocol for ING2 antibody 11560-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cell Proteomics Human family with sequence similarity 60 member A (FAM60A) protein: a new subunit of the Sin3 deacetylase complex. | ||
Chem Biol Deacetylase inhibitors dissociate the histone-targeting ING2 subunit from the Sin3 complex.
| ||
Cancers (Basel) Antithetic hTERT Regulation by Androgens in Prostate Cancer Cells: hTERT Inhibition Is Mediated by the ING1 and ING2 Tumor Suppressors.
| ||
Front Cell Dev Biol ING2 Controls Mitochondrial Respiration via Modulating MRPL12 Ubiquitination in Renal Tubular Epithelial Cells.
| ||
Cancer Lett Decreased expression of ING2 gene and its clinicopathological significance in hepatocellular carcinoma. | ||
Biochem J Histone H3 N-terminal mimicry drives a novel network of methyl-effector interactions. |