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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ING3-specific Polyclonal antibody

ING3-specific Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 16187-1-AP

Synonyms

Eaf4, ING2, ING3, p47ING3

Validation Data Gallery

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  • Western Blot (WB) analysis of human kidney tissue using ING3-specific Polyclonal antibody (16187-1-AP)

    WB analysis of human kidney using 16187-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 16187-1-AP (ING3-specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using ING3-specific Polyclonal antibody (16187-1-AP)

    WB analysis of mouse liver using 16187-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 16187-1-AP (ING3-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using ING3-specific Polyclonal antibody (16187-1-AP)

    WB analysis of mouse kidney using 16187-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16187-1-AP (ING3-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse heart tissue using ING3-specific Polyclonal antibody (16187-1-AP)

    WB analysis of mouse heart using 16187-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 16187-1-AP (ING3-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse kidney tissue using ING3-specific Polyclonal antibody (16187-1-AP)

    IP experiment of mouse kidney using 16187-1-AP

    IP result of anti-ING3-specific (IP:16187-1-AP, 4ug; Detection:16187-1-AP 1:500) with mouse kidney tissue lysate 4000ug.

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Tested Applications

Positive WB detected inhuman kidney tissue, mouse heart tissue, mouse kidney tissue, mouse liver tissue
Positive IP detected inmouse kidney tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16187-1-AP targets ING3-specific in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name inhibitor of growth family, member 3
Calculated molecular weight 47 kDa
Observed molecular weight 46 kDa
GenBank accession numberNM_019071
Gene symbol ING3
Gene ID (NCBI) 54556
RRIDAB_2264993
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Members of inhibitor of growth (ING) family function in inhibiting cell growth and inducing apoptosis. They are sequence homologous proteins. ING3 can activate p53 trans-activated promoters, including promoters of p21/waf1 and bax. This antibody is specifically against p47ING3.

Protocols

Product Specific Protocols
WB protocol for ING3-specific antibody 16187-1-APDownload protocol
IP protocol for ING3-specific antibody 16187-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols