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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ING4 Monoclonal antibody (67754-1-Ig)

    WB analysis using 67754-1-Ig (same clone as 67754-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67754-1-Ig (ING4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 67754-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig brain tissue using ING4 Monoclonal antibody (67754-1-Ig)

    WB analysis of pig brain using 67754-1-Ig (same clone as 67754-1-PBS)

    pig brain tissue were subjected to SDS PAGE followed by western blot with 67754-1-Ig (ING4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67754-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rabbit brain tissue using ING4 Monoclonal antibody (67754-1-Ig)

    WB analysis of rabbit brain using 67754-1-Ig (same clone as 67754-1-PBS)

    rabbit brain tissue were subjected to SDS PAGE followed by western blot with 67754-1-Ig (ING4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67754-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat brain tissue using ING4 Monoclonal antibody (67754-1-Ig)

    WB analysis of rat brain using 67754-1-Ig (same clone as 67754-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 67754-1-Ig (ING4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67754-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using ING4 Monoclonal antibody (67754-1-Ig)

    IHC staining of human colon cancer using 67754-1-Ig (same clone as 67754-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67754-1-Ig (ING4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67754-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using ING4 Monoclonal antibody (67754-1-Ig)

    IHC staining of human cervical cancer using 67754-1-Ig (same clone as 67754-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67754-1-Ig (ING4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67754-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using ING4 Monoclonal antibody (67754-1-Ig)

    IHC staining of human cervical cancer using 67754-1-Ig (same clone as 67754-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67754-1-Ig (ING4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67754-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using ING4 Monoclonal antibody (67754-1-Ig)

    IHC staining of human colon cancer using 67754-1-Ig (same clone as 67754-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67754-1-Ig (ING4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67754-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human colon cancer tissue using ING4 Monoclonal antibody (67754-1-Ig)

    IF Staining of human colon cancer using 67754-1-Ig (same clone as 67754-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using ING4 antibody (67754-1-Ig, Clone: 1A12A3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67754-1-PBS in a different storage buffer formulation.

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ING4 Monoclonal antibody, PBS Only

ING4 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 67754-1-PBS
Clone No.1A12A3

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig, rabbit, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

ING4, Inhibitor of growth protein 4, my036, p29ING4

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67754-1-PBS targets ING4 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with Human, pig, rabbit, rat samples.

Tested Reactivity Human, pig, rabbit, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ING4 fusion protein Ag4610 相同性解析による交差性が予測される生物種
Full Name inhibitor of growth family, member 4
Calculated molecular weight 29 kDa
Observed molecular weight29 kDa
GenBank accession numberBC007781
Gene Symbol ING4
Gene ID (NCBI) 51147
RRIDAB_2918522
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9UNL4
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

ING4, also named as p29ING4, belongs to the ING family. It is a component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. It may inhibit tumor progression by modulating the transcriptional output of signaling pathways which regulate cell proliferation. ING4 can suppress brain tumor angiogenesis through transcriptional repression of RELA/NFKB3 target genes when complexed with RELA. It may also specifically suppress loss of contact inhibition elicited by activated oncogenes such as MYC. Represses hypoxia inducible factor's (HIF) activity by interacting with HIF prolyl hydroxylase 2 (EGLN1). ING4 is a tumor suppressor gene that interacts with NFkB and represses its transcriptional activity. Several lines of evidence suggest that the tumor suppressor gene ING4, NFkB and its target genes matrix metalloproteases MMP-2, MMP-9 and u-PA are critically involved in tumor invasion.