ING4-specific Polyclonal antibody

Name: ING4-specific Polyclonal antibody
Brand: Proteintech
SKU: 16188-1-AP

ING4-specific Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 16188-1-AP

Synonyms

ING4, Inhibitor of growth protein 4, my036, p29ING4

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis of Jurkat using 16188-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 16188-1-AP (ING4-specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 16188-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 16188-1-AP (ING4-specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse colon using 16188-1-AP
mouse colon tissue were subjected to SDS PAGE followed by western blot with 16188-1-AP (ING4-specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse colon using 16188-1-AP
mouse colon tissue were subjected to SDS PAGE followed by western blot with 16188-1-AP (ING4-specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 16188-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 16188-1-AP (ING4-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 16188-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 16188-1-AP (ING4-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 16188-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 16188-1-AP (ING4-specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 16188-1-AP
IP result of anti-ING4-specific (IP:16188-1-AP, 3ug; Detection:16188-1-AP 1:300) with HeLa cells lysate 2500ug.
IHC staining of human brain using 16188-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 16188-1-AP (ING4-specific antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human brain using 16188-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 16188-1-AP (ING4-specific antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human liver cancer using 16188-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16188-1-AP (ING4-specific Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human liver cancer using 16188-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16188-1-AP (ING4-specific Antibody) at dilution of 1:50 (under 40x lens).
IF Staining of HepG2 using 16188-1-AP
Immunofluorescent analysis of HepG2 cells using 16188-1-AP (ING4-specific antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Cat No. 16188-1-AP

Size/Concentration

ご購入について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	Jurkat cells, HEK-293 cells, HeLa cells, mouse colon tissue
Positive IP detected in	HeLa cells
Positive IHC detected in	human brain tissue, human liver cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 2 publications below
IHC	See 5 publications below
IF	See 1 publications below

Product Information

16188-1-AP targets ING4-specific in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide 相同性解析による交差性が予測される生物種
Full Name	inhibitor of growth family, member 4
Calculated molecular weight	29 kDa
Observed molecular weight	29-32 kDa
GenBank accession number	NM_001127582
Gene symbol	ING4
Gene ID (NCBI)	51147
RRID	AB_2127798
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ING4, also named as p29ING4, belongs to the ING family. It is a component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. It may inhibit tumor progression by modulating the transcriptional output of signaling pathways which regulate cell proliferation. ING4 can suppress brain tumor angiogenesis through transcriptional repression of RELA/NFKB3 target genes when complexed with RELA. It may also specifically suppress loss of contact inhibition elicited by activated oncogenes such as MYC. Represses hypoxia inducible factor's (HIF) activity by interacting with HIF prolyl hydroxylase 2 (EGLN1). ING4 is a tumor suppressor gene that interacts with NFkB and represses its transcriptional activity. Several lines of evidence suggest that the tumor suppressor gene ING4, NFkB and its target genes matrix metalloproteases MMP-2, MMP-9 and u-PA are critically involved in tumor invasion. This antibody is specifically against p29ING4.

Protocols

Product Specific Protocols
WB protocol for ING4-specific antibody 16188-1-AP	Download protocol
IHC protocol for ING4-specific antibody 16188-1-AP	Download protocol
IF protocol for ING4-specific antibody 16188-1-AP	Download protocol
IP protocol for ING4-specific antibody 16188-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Oncogene ING4 regulates a secretory phenotype in primary fibroblasts with dual effects on cell proliferation and tumor growth. Authors - Moreno A A View Article
human	IHC	PLoS One Novel multiple markers to distinguish melanoma from dysplastic nevi. Authors - Zhang Guohong G View Article
		Am J Physiol Heart Circ Physiol Carvedilol-responsive microRNAs, miR-199a-3p and -214 protect cardiomyocytes from simulated ischemia-reperfusion injury. Authors - Kyoung-Mi Park View Article
human	WB,IHC,IF	Oral Oncol Downregulation and translocation of nuclear ING4 is correlated with tumorigenesis and progression of head and neck squamous cell carcinoma. Authors - Li Xiao-han XH View Article
human	IHC	Acta Histochem Inhibitor of growth 4 (ING4) inhibits hypoxia-induced EMT by decreasing HIF-1α and snail in HK2 cells. Authors - Lingling Lu View Article
human	IHC	Mol Med Rep PMID: 27484725 Authors - Xin Ren View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ING4-specific Polyclonal antibody

ING4-specific Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis of Jurkat using 16188-1-AP

WB analysis of Jurkat using 16188-1-AP

WB analysis of mouse colon using 16188-1-AP

WB analysis of mouse colon using 16188-1-AP

WB analysis of HeLa using 16188-1-AP

WB analysis of HEK-293 using 16188-1-AP

WB analysis of Jurkat using 16188-1-AP

IP experiment of HeLa using 16188-1-AP

IHC staining of human brain using 16188-1-AP

IHC staining of human brain using 16188-1-AP

IHC staining of human liver cancer using 16188-1-AP

IHC staining of human liver cancer using 16188-1-AP

IF Staining of HepG2 using 16188-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

ING4 regulates a secretory phenotype in primary fibroblasts with dual effects on cell proliferation and tumor growth.

Novel multiple markers to distinguish melanoma from dysplastic nevi.

Carvedilol-responsive microRNAs, miR-199a-3p and -214 protect cardiomyocytes from simulated ischemia-reperfusion injury.

Downregulation and translocation of nuclear ING4 is correlated with tumorigenesis and progression of head and neck squamous cell carcinoma.

Inhibitor of growth 4 (ING4) inhibits hypoxia-induced EMT by decreasing HIF-1α and snail in HK2 cells.

PMID: 27484725