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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using INO80 Recombinant antibody (83117-4-RR)

    IF Staining of HepG2 using 83117-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using INO80 antibody (83117-4-RR, Clone: 230440E9 ) at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using INO80 Recombinant antibody (83117-4-RR)

    IF Staining of HepG2 using 83117-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using INO80 antibody (83117-4-RR, Clone: 230440E9 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using INO80 Recombinant antibody (83117-4-RR)

    FC experiment of HeLa using 83117-4-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Ino80 Recombinant Antibody (83117-4-RR, Clone:230440E9) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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INO80 Recombinant antibody

INO80 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 83117-4-RR
Clone No.230440E9

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF/ICC, FC (Intra), ELISA

Synonyms

hINO80, INO80, INO80 homolog (S. cerevisiae), INO80A, INOC1, KIAA1259

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83117-4-RR targets INO80 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name INO80 homolog (S. cerevisiae)
Calculated molecular weight 1556 aa, 177 kDa
GenBank accession numberBC146785
Gene Symbol INO80
Gene ID (NCBI) 54617
RRIDAB_3670830
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9ULG1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for INO80 antibody 83117-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols