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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

INPP4B Polyclonal antibody

INPP4B Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15890-1-AP

Synonyms

INPP4B

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using INPP4B Polyclonal antibody (15890-1-AP)

    WB analysis using 15890-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15890-1-AP (INPP4B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using INPP4B Polyclonal antibody (15890-1-AP)

    IHC staining of human lung cancer using 15890-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15890-1-AP (INPP4B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using INPP4B Polyclonal antibody (15890-1-AP)

    IHC staining of human lung cancer using 15890-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15890-1-AP (INPP4B antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inMCF-7 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15890-1-AP targets INPP4B in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen INPP4B fusion protein Ag8676 相同性解析による交差性が予測される生物種
Full Name inositol polyphosphate-4-phosphatase, type II, 105kDa
Calculated molecular weight 53 aa, 6 kDa
Observed molecular weight 100-110 kDa
GenBank accession numberBC005273
Gene symbol INPP4B
Gene ID (NCBI) 8821
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

INPP4B (Inositol polyphosphate 4-phosphatase type II) can catalyze the hydrolysis of the 4-position phosphate of phosphatidylinositol 3,4-bisphosphate, inositol 1,3,4-trisphosphate and inositol 3,4-trisphosphate (PMID: 24070612; 24591580). It also Plays a role in the late stages of macropinocytosis by dephosphorylating phosphatidylinositol 3,4-bisphosphate in membrane ruffles (PMID: 24591580).

Protocols

Product Specific Protocols
WB protocol for INPP4B antibody 15890-1-APDownload protocol
IHC protocol for INPP4B antibody 15890-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols