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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using INPP4B Recombinant antibody (85151-4-RR)

    WB analysis using 85151-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85151-4-RR (INPP4B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using INPP4B Recombinant antibody (85151-4-RR)

    IF Staining of MCF-7 using 85151-4-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using INPP4B antibody (85151-4-RR, Clone: 242837H2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of K562 using INPP4B Recombinant antibody (85151-4-RR)

    FC experiment of K562 using 85151-4-RR

    1x10^6 K562 were intracellularly stained with 0.25 ug INPP4B Recombinant antibody (85151-4-RR, Clone:242837H2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 85151-4-RR

    Affinity and Kinetic Characterization of 85151-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85151-4-RR against Human INPP4B were performed. The affinity constant is 0.563 nM.

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INPP4B Recombinant antibody

INPP4B Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 85151-4-RR
Clone No.242837H2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

242837H2, EC:3.1.3.66, Inositol polyphosphate 4-phosphatase type II, Type II inositol 3,4-bisphosphate 4-phosphatase

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inLNCaP cells, A549 cells, HeLa cells, MCF-7 cells
Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inK562

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85151-4-RR targets INPP4B in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen INPP4B fusion protein Ag8676 相同性解析による交差性が予測される生物種
Full Name inositol polyphosphate-4-phosphatase, type II, 105kDa
Calculated molecular weight 53 aa, 6 kDa
Observed molecular weight 100-110 kDa
GenBank accession numberBC005273
Gene Symbol INPP4B
Gene ID (NCBI) 8821
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO15327
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

INPP4B (Inositol polyphosphate 4-phosphatase type II) can catalyze the hydrolysis of the 4-position phosphate of phosphatidylinositol 3,4-bisphosphate, inositol 1,3,4-trisphosphate and inositol 3,4-trisphosphate (PMID: 24070612; 24591580). It also Plays a role in the late stages of macropinocytosis by dephosphorylating phosphatidylinositol 3,4-bisphosphate in membrane ruffles (PMID: 24591580).

Protocols

Product Specific Protocols
WB protocol for INPP4B antibody 85151-4-RRDownload protocol
IF protocol for INPP4B antibody 85151-4-RRDownload protocol
FC protocol for INPP4B antibody 85151-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols