Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse spleen tissue, HeLa cells, human placenta tissue |
| Positive IHC detected in | mouse pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
15141-1-AP targets INPP5B in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | INPP5B fusion protein Ag7315 相同性解析による交差性が予測される生物種 |
| Full Name | inositol polyphosphate-5-phosphatase, 75kDa |
| Calculated molecular weight | 113 kDa |
| Observed molecular weight | 75 kDa |
| GenBank accession number | BC058932 |
| Gene Symbol | INPP5B |
| Gene ID (NCBI) | 3633 |
| RRID | AB_2126133 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P32019 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
INPP5B(Type II inositol 1,4,5-trisphosphate 5-phosphatase) is also named as OCRL2,5PTase, 75 kDa inositol polyphosphate-5-phosphatase and belongs to the inositol 1,4,5-trisphosphate 5-phosphatase type II family. This protein, originally described in platelets, is one of at least three known enzymes capable of dephosphorylating inositol-1,4,5-trisphosphate (IP3) to inositol-1,4-bisphosphate (IP2)(PMID:8125013). It has 4 isoforms produced by alternative splicing.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for INPP5B antibody 15141-1-AP | Download protocol |
| IHC protocol for INPP5B antibody 15141-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Am Soc Nephrol Effects of Proximal Tubule Shortening on Protein Excretion in a Lowe Syndrome Model. | ||
Cancer Cell Int Decreased INPP5B expression predicts poor prognosis in lung adenocarcinoma. | ||
PLoS One Compensatory Role of Inositol 5-Phosphatase INPP5B to OCRL in Primary Cilia Formation in Oculocerebrorenal Syndrome of Lowe. | ||
Biochim Biophys Acta Mol Basis Dis Integrated application of transcriptome and metabolomics reveals potential therapeutic targets for the polarization of atherosclerotic macrophages | ||
J Biol Chem Comparative proximity biotinylation implicates the small GTPase RAB18 in sterol mobilization and biosynthesis |