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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

INPP5E Polyclonal antibody

INPP5E Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 29353-1-AP

Synonyms

INPP5E, PPI5PIV

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using INPP5E Polyclonal antibody (29353-1-AP)

    WB analysis using 29353-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29353-1-AP (INPP5E antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of ARPE-19 cells using INPP5E Polyclonal antibody (29353-1-AP)

    IF Staining of ARPE-19 using 29353-1-AP

    Immunofluorescent analysis of (4% PFA) fixed ARPE-19 cells using INPP5E antibody (29353-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of ARPE-19 cells using INPP5E Polyclonal antibody (29353-1-AP)

    IF Staining of ARPE-19 using 29353-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed ARPE-19 cells using INPP5E antibody (29353-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHEK-293 cells, mouse testis tissue, rat testis tissue
Positive IF/ICC detected inARPE-19 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29353-1-AP targets INPP5E in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen INPP5E fusion protein Ag30305 相同性解析による交差性が予測される生物種
Full Name inositol polyphosphate-5-phosphatase, 72 kDa
Calculated molecular weight 644 aa, 70 kDa
Observed molecular weight70 kDa
GenBank accession numberBC028032
Gene symbol INPP5E
Gene ID (NCBI) 56623
RRIDAB_3086123
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

INPP5E(72 kDa inositol polyphosphate 5-phosphatase) converts phosphatidylinositol 3,4,5-trisphosphate (PtdIns 3,4,5-P3) to PtdIns-P2.In mouse, highest protein expression was in brain, heart, and testis, with lower expression in thymus and lung, and very little expression in kidney, spleen, and liver(PMID:10764818). Defects in INPP5E are the cause of Joubert syndrome type 1 (JBTS1) and mental retardation-truncal obesity-retinal dystrophy-micropenis (MORMS)(PMID:19668215). It has 2 isoforms with the molecular mass of 66 kDa and 70 kDa.

Protocols

Product Specific Protocols
WB protocol for INPP5E antibody 29353-1-APDownload protocol
IF protocol for INPP5E antibody 29353-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols