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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

INPP5F Polyclonal antibody

INPP5F Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 27240-1-AP

Synonyms

hSAC2, INPP5F, KIAA0966, MSTP007, MSTP047, MSTPO47, SAC2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using INPP5F Polyclonal antibody (27240-1-AP)

    WB analysis using 27240-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27240-1-AP (INPP5F antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of MCF-7 cells using INPP5F Polyclonal antibody (27240-1-AP)

    IP experiment of MCF-7 using 27240-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 27240-1-AP (INPP5F antibody) at dilution of 1:500.

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Tested Applications

Positive WB detected inHEK-293 cells, MCF-7 cells
Positive IP detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27240-1-AP targets INPP5F in WB, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen INPP5F fusion protein Ag25687 相同性解析による交差性が予測される生物種
Full Name inositol polyphosphate-5-phosphatase F
Calculated molecular weight 1132 aa, 128 kDa
Observed molecular weight128-140 kDa
GenBank accession numberBC111493
Gene symbol INPP5F
Gene ID (NCBI) 22876
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

INPP5F can negatively regulates STAT3 signaling pathway through inhibition of STAT3 phosphorylation and translocation to the nucleus.

Protocols

Product Specific Protocols
WB protocol for INPP5F antibody 27240-1-APDownload protocol
IP protocol for INPP5F antibody 27240-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols