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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

INPP5J Polyclonal antibody

INPP5J Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 29949-1-AP

Synonyms

Phosphatidylinositol 1,4,5-trisphosphate 5-phosphatase, Phosphatidylinositol 1,3,4,5-tetrakisphosphate 5-phosphatase, INPP5, Inositol polyphosphate 5-phosphatase J, EC:3.1.3.56

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using INPP5J Polyclonal antibody (29949-1-AP)

    WB analysis using 29949-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29949-1-AP (INPP5J antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using INPP5J Polyclonal antibody (29949-1-AP)

    IF Staining of HeLa using 29949-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using INPP5J antibody (29949-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inA549 cells, HeLa cells, MCF-7 cells, mouse heart tissue
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29949-1-AP targets INPP5J in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen INPP5J fusion protein Ag29242 相同性解析による交差性が予測される生物種
Full Name inositol polyphosphate-5-phosphatase J
Calculated molecular weight 1006 aa, 107 kDa
Observed molecular weight70 kDa
GenBank accession numberBC109288
Gene symbol INPP5J
Gene ID (NCBI) 27124
RRIDAB_2918353
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for INPP5J antibody 29949-1-APDownload protocol
IF protocol for INPP5J antibody 29949-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols