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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using INPP5K Polyclonal antibody (15098-1-AP)

    WB analysis of HEK-293 using 15098-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 15098-1-AP (INPP5K antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse heart tissue using INPP5K Polyclonal antibody (15098-1-AP)

    WB analysis of mouse heart using 15098-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 15098-1-AP (INPP5K antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using INPP5K Polyclonal antibody (15098-1-AP)

    IHC staining of human kidney using 15098-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 15098-1-AP (INPP5K antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using INPP5K Polyclonal antibody (15098-1-AP)

    IHC staining of human kidney using 15098-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 15098-1-AP (INPP5K antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using INPP5K Polyclonal antibody (15098-1-AP)

    IHC staining of human heart using 15098-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 15098-1-AP (INPP5K antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using INPP5K Polyclonal antibody (15098-1-AP)

    IHC staining of human heart using 15098-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 15098-1-AP (INPP5K antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using INPP5K Polyclonal antibody (15098-1-AP)

    IF Staining of HEK-293 using 15098-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 15098-1-AP (INPP5K antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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INPP5K Polyclonal antibody

INPP5K Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 15098-1-AP
Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Phosphatidylinositol-4,5-bisphosphate 5-phosphatase, Phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase, Inositol polyphosphate 5-phosphatase K, EC:3.1.3.86, EC:3.1.3.56

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, mouse heart tissue
Positive IHC detected inhuman kidney tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15098-1-AP targets INPP5K in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen INPP5K fusion protein Ag7192 相同性解析による交差性が予測される生物種
Full Name inositol polyphosphate-5-phosphatase K
Calculated molecular weight 51 kDa
Observed molecular weight 45-51 kDa
GenBank accession numberBC004362
Gene Symbol INPP5K
Gene ID (NCBI) 51763
RRIDAB_2126365
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BT40
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for INPP5K antibody 15098-1-APDownload protocol
IHC protocol for INPP5K antibody 15098-1-APDownload protocol
IF protocol for INPP5K antibody 15098-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Chem Biol

Triplexed Affinity Reagents to Sample the Mammalian Inositol Pyrophosphate Interactome.

Authors - David Furkert
View Article
human,mouseWB,IF

Front Mol Neurosci

Knockdown of INPP5K compromises the differentiation of N2A cells

Authors - Annamaria Manzolillo
View Article
mouseWB

Exp Neurol

METTL5-mediated 18S rRNA m6A modification promotes corticospinal tract sprouting after unilateral traumatic brain injury

Authors - Zhenpeng Li
View Article