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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

INPPL1 Polyclonal antibody

INPPL1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26678-1-AP

Synonyms

INPPL 1, INPPL1, Protein 51C, SHIP 2, SHIP2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using INPPL1 Polyclonal antibody (26678-1-AP)

    WB analysis using 26678-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26678-1-AP (INPPL1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using INPPL1 Polyclonal antibody (26678-1-AP)

    IP experiment of HeLa using 26678-1-AP

    IP result of anti-INPPL1 (IP:26678-1-AP, 4ug; Detection:26678-1-AP 1:800) with HeLa cells lysate 2520 ug.

  • Immunohistochemistry (IHC) staining of mouse stomach tissue using INPPL1 Polyclonal antibody (26678-1-AP)

    IHC staining of mouse stomach using 26678-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 26678-1-AP (INPPL1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse stomach tissue using INPPL1 Polyclonal antibody (26678-1-AP)

    IHC staining of mouse stomach using 26678-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 26678-1-AP (INPPL1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHeLa cells, NIH/3T3 cells
Positive IP detected inHeLa cells
Positive IHC detected inmouse stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26678-1-AP targets INPPL1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen INPPL1 fusion protein Ag24790 相同性解析による交差性が予測される生物種
Full Name inositol polyphosphate phosphatase-like 1
Calculated molecular weight 1258 aa, 139 kDa
Observed molecular weight150 kDa
GenBank accession numberBC140853
Gene symbol INPPL1
Gene ID (NCBI) 3636
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for INPPL1 antibody 26678-1-APDownload protocol
IHC protocol for INPPL1 antibody 26678-1-APDownload protocol
IP protocol for INPPL1 antibody 26678-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols