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KD/KO Validated

INSIG1 Polyclonal antibody

Name: INSIG1 Polyclonal antibody
Brand: Proteintech
SKU: 22115-1-AP

INSIG1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 22115-1-AP

Synonyms

CL 6, INSIG 1, INSIG1, insulin induced gene 1, Insulin induced gene 1 protein

Validation Data Gallery

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AllWBIHC

WB analysis of A549 using 22115-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 22115-1-AP (INSIG1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 22115-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 22115-1-AP (INSIG1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 22115-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 22115-1-AP (INSIG1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 22115-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 22115-1-AP (INSIG1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of SGC-7901 using 22115-1-AP
SGC-7901 cells were subjected to SDS PAGE followed by western blot with 22115-1-AP (INSIG1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human liver using 22115-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 22115-1-AP (INSIG1 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human liver using 22115-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 22115-1-AP (INSIG1 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human kidney using 22115-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 22115-1-AP (INSIG1 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human kidney using 22115-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 22115-1-AP (INSIG1 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human kidney using 22115-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 22115-1-AP (INSIG1 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human kidney using 22115-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 22115-1-AP (INSIG1 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human liver using 22115-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 22115-1-AP (INSIG1 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human liver using 22115-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 22115-1-AP (INSIG1 antibody) at dilution of 1:50 (under 40x lens).

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Cat No. 22115-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	A549 cells, HepG2 cells, SGC-7901 cells, MCF-7 cells
Positive IHC detected in	human liver tissue, human kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 6 publications below
IHC	See 1 publications below
IF	See 3 publications below

Product Information

The immunogen of 22115-1-AP is INSIG1 Fusion Protein expressed in E. coli.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	INSIG1 fusion protein Ag17420 相同性解析による交差性が予測される生物種
Full Name	insulin induced gene 1
Calculated molecular weight	277 aa, 30 kDa
Observed molecular weight	28-31 kDa, 66-70 kDa
GenBank accession number	BC001880
Gene symbol	INSIG1
Gene ID (NCBI)	3638
RRID	AB_11183051
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Insulin-induced genes (INSIGs), which consist of two isoforms (INSIG1 and INSIG2), are representative endoplasmic reticulum (ER)-resident oxysterol-binding proteins (OSBPs). INSIG1 is part of negative regulatory feedback, acting as a brake on SREBP transcriptional function (PMID: 23919961). INSIG1 also has a secondary function mediating sterolaccelerated proteolytic degradation of HMG CoA reductase, thus impacting the mevalonate pathway. Suppression of INSIG1 function promotes hepatic lipid remodelling and restrains NASH (non-alcoholic steatohepatitis) progression(PMID: 33722690).

Protocols

Product Specific Protocols
WB protocol for INSIG1 antibody 22115-1-AP	Download protocol
IHC protocol for INSIG1 antibody 22115-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IF	Nature The gluconeogenic enzyme PCK1 phosphorylates INSIG1/2 for lipogenesis. Authors - Daqian Xu KD Validated View Article
	WB	Transl Psychiatry A potential mechanism underlying atypical antipsychotics-induced lipid disturbances. Authors - H L Cai View Article
	WB	Eur Neuropsychopharmacol Vitamin D deficiency exacerbates atypical antipsychotic-induced metabolic side effects in rats: Involvement of the INSIG/SREBP pathway. Authors - Ruili Dang View Article
	WB	Mol Nutr Food Res (-)-Epicatechin regulates blood lipids and attenuates hepatic steatosis in rats fed high-fat diet(†). Authors - Hui Cheng View Article
	WB,IHC	J Physiol Biochem Renal tubule ectopic lipid deposition in diabetic kidney disease rat model and in vitro mechanism of leptin intervention. Authors - Shasha Liu View Article
human	IF	JCI Insight Hypomorphic ASGR1 modulates lipid homeostasis via INSIG1-mediated SREBP signaling suppression. Authors - Yingying Xu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

INSIG1 Polyclonal antibody

INSIG1 Polyclonal Antibody for WB, IHC, ELISA

Validation Data Gallery

WB analysis of A549 using 22115-1-AP

WB analysis of MCF-7 using 22115-1-AP

WB analysis of A549 using 22115-1-AP

WB analysis of HepG2 using 22115-1-AP

WB analysis of SGC-7901 using 22115-1-AP

IHC staining of human liver using 22115-1-AP

IHC staining of human liver using 22115-1-AP

IHC staining of human kidney using 22115-1-AP

IHC staining of human kidney using 22115-1-AP

IHC staining of human kidney using 22115-1-AP

IHC staining of human kidney using 22115-1-AP

IHC staining of human liver using 22115-1-AP

IHC staining of human liver using 22115-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

The gluconeogenic enzyme PCK1 phosphorylates INSIG1/2 for lipogenesis.

A potential mechanism underlying atypical antipsychotics-induced lipid disturbances.

Vitamin D deficiency exacerbates atypical antipsychotic-induced metabolic side effects in rats: Involvement of the INSIG/SREBP pathway.

(-)-Epicatechin regulates blood lipids and attenuates hepatic steatosis in rats fed high-fat diet(†).

Renal tubule ectopic lipid deposition in diabetic kidney disease rat model and in vitro mechanism of leptin intervention.

Hypomorphic ASGR1 modulates lipid homeostasis via INSIG1-mediated SREBP signaling suppression.