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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using INSL5 Polyclonal antibody (27697-1-AP)

    WB analysis using 27697-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27697-1-AP (INSL5 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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INSL5 Polyclonal antibody

INSL5 Polyclonal Antibody for WB, ELISA
Cat No. 27697-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, ELISA

Synonyms

INSL5, insulin like 5, Insulin like peptide 5, Insulin like peptide INSL5, PRO182, UNQ156

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse testis tissue, rat testis

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27697-1-AP targets INSL5 in WB, ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen INSL5 fusion protein Ag26833 相同性解析による交差性が予測される生物種
Full Name insulin-like 5
Observed molecular weight12-15 kDa
GenBank accession numberBC101646
Gene Symbol INSL5
Gene ID (NCBI) 10022
RRIDAB_3085985
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y5Q6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Insulin-like peptide 5 (INSL5), also named as UNQ156/PRO182, is a member of the relaxin/insulin superfamily and presents a tertiary structure similar to that of other insulin family members. It is located outside the cell membranes and highly expressed in rectum with lower levels in uterus and ascending and descending colon (PMID: 10458910). The calculated molecular weight of INSL5 is 15 kDa. A recent report suggested an important role for Isnl5 in the regulation of insulin secretion and b-cell homeostasis. Other reports identified that colonic Isnl5 expression is reduced by the gut microbiota and energy availability. In human diseases, INSL5 has been identified recently in colonic tissue and neuroendocrine tumors, but its specific functions in tumors remain to be elucidated(PMID: 32657028).

Protocols

Product Specific Protocols
WB protocol for INSL5 antibody 27697-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols