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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

INSM1 Polyclonal antibody

INSM1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 55477-1-AP

Synonyms

IA-1, Zinc finger protein IA 1, insulinoma associated 1, INSM 1, IA1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using INSM1 Polyclonal antibody (55477-1-AP)

    WB analysis using 55477-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 55477-1-AP (INSM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55477-1-AP targets INSM1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name insulinoma-associated 1
Calculated molecular weight 53 kDa
Observed molecular weight53-60 kDa
GenBank accession numberNM_002196
Gene symbol INSM1
Gene ID (NCBI) 3642
RRIDAB_3086454
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Insulinoma-associated-1 (INSM1) is a zinc-finger transcription factor that was originally identified in a human insulinoma subtraction library. INSM1 plays a cardinal role as a transcription factor in the differentiation of embryonic neuroendocrine cells. In situ hybridization studies of human tissues have shown the presence of the INSM1 transcript across various brain regions, as well as in endocrine organs such as the pancreas, adrenal gland, thymus, thyroid, and the endocrine cells of the gastrointestinal tract .(PMID: 34943408, PMID: 36290282)

Protocols

Product Specific Protocols
WB protocol for INSM1 antibody 55477-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols