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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using INSR Recombinant antibody (84467-2-RR)

    WB analysis using 84467-2-RR (same clone as 84467-2-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 84467-2-RR (INSR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84467-2-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01349-2

    Sandwich ELISA standard curve of MP01349-2

    Sandwich ELISA standard curve of MP01349-2, Human INSR Recombinant Matched Antibody Pair - PBS only. 84467-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard RP00007. 84467-2-PBS was HRP conjugated as the detection antibody. Range: 1.56-100 ng/mL.

  • Sample test of MP01349-2

    Sample test of MP01349-2

    The mean INSR concentration was determined to be 72.33 ng/mL in HEK-293 cell extract based on a 1.50 mg/mL extract load and 87.72 ng/mL in HT-1080 cell extract based on a 2.50 mg/mL extract load.

  • Cytometric bead array standard curve of MP01349-1

    Cytometric bead array standard curve of MP01349-1

    Cytometric bead array standard curve of MP01349-1, INSR Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84467-2-PBS. Detection antibody: 84467-1-PBS. Standard: RP00007. Range: 1.56-100 ng/mL.

  • Affinity and Kinetic Characterization of 84467-2-RR

    Affinity and Kinetic Characterization of 84467-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84467-2-RR against Human INSR were performed. The affinity constant is 0.189 nM.

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INSR Recombinant antibody, PBS Only (Capture/Detector)

INSR Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 84467-2-PBS
Clone No.241687H7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

IR, insulin receptor, HHF5, EC:2.7.10.1, CD220

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84467-2-PBS targets INSR as part of a matched antibody pair:

MP01349-1: 84467-2-PBS capture and 84467-1-PBS detection (validated in Cytometric bead array)

MP01349-2: 84467-1-PBS capture and 84467-2-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant Protein 相同性解析による交差性が予測される生物種
Full Name INSR
Observed molecular weight95 kDa
GenBank accession numberBC117172
Gene Symbol INSR
Gene ID (NCBI) 3643
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP06213
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The biological effects of INS are mediated by a membrane-spanning cell surface receptor that has been shown to consist of disulfide-linked alpha-subunits (135 kDa) and beta-subunits (95 kDa) that are cleaved products of a common precursor. The binding of INS to the receptor extracellular domain results in intracellular activation of a tyrosine-specific kinase activity and the generation of signals that determine the cellular response (PMID: 2369896).