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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human INSR/CD220 Rabbit Recombinant Antibody

INSR/CD220 Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

241687A10

Cat no : 98227-1-RR

Synonyms

INSR, insulin receptor, HHF5, EC:2.7.10.1, CD220

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human INSR/CD220 Rabbit Recombinant Antibody (98227-1-RR)

    FC experiment of human PBMCs using 98227-1-RR

    1x10^6 FC receptor blocked human PBMCs were surface stained with 0.25 ug Anti-Human INSR/CD220 Rabbit Recombinant Antibody (98227-1-RR, Clone: 241687A10) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with 0.25 ug CoraLite® Plus 647 Anti-Human CD14 Rabbit Recombinant Antibody (CL647-98040, Clone: 230332D7). Cells were not fixed.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human INSR/CD220 Rabbit Recombinant Antibody (98227-1-RR)

    FC experiment of human PBMCs using 98227-1-RR

    1x10^6 FC receptor blocked human PBMCs were surface stained with 0.25 ug Anti-Human INSR/CD220 Rabbit Recombinant Antibody (98227-1-RR, Clone:241687A10) (red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

  • Affinity and Kinetic Characterization of 98227-1-RR

    Affinity and Kinetic Characterization of 98227-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98227-1-RR against Human INSR/CD220 were performed. The affinity constant is 52.4 pM.

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Tested Applications

Positive FC detected inFC receptor blocked human PBMCs

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98227-1-RR targets INSR/CD220 in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name INSR
GenBank accession numberBC117172
Gene symbol INSR
Gene ID (NCBI) 3643
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

Background Information

The biological effects of INS are mediated by a membrane-spanning cell surface receptor that has been shown to consist of disulfide-linked alpha-subunits (135 kDa) and beta-subunits (95 kDa) that are cleaved products of a common precursor. The binding of INS to the receptor extracellular domain results in intracellular activation of a tyrosine-specific kinase activity and the generation of signals that determine the cellular response (PMID: 2369896).

Protocols

Product Specific Protocols
FC protocol for INSR/CD220 antibody 98227-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols