• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

INTS12 Polyclonal antibody

INTS12 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16455-1-AP

Synonyms

SBBI22, PHF22, Int12

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HepG2 cells using INTS12 Polyclonal antibody (16455-1-AP)

    WB analysis of HepG2 using 16455-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16455-1-AP (INTS12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using INTS12 Polyclonal antibody (16455-1-AP)

    WB analysis of HepG2 using 16455-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16455-1-AP (INTS12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach tissue using INTS12 Polyclonal antibody (16455-1-AP)

    IHC staining of human stomach using 16455-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 16455-1-AP (INTS12 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

☆大好評トライアルサイズ ¥20,000～！ >>> 一次抗体特別価格キャンペーン開催中！

Tested Applications

Positive WB detected inHepG2 cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

16455-1-AP targets INTS12 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen INTS12 fusion protein Ag9403 相同性解析による交差性が予測される生物種
Full Name integrator complex subunit 12
Calculated molecular weight 462 aa, 49 kDa
Observed molecular weight 49 kDa
GenBank accession numberBC014442
Gene symbol INTS12
Gene ID (NCBI) 57117
RRIDAB_2127263
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for INTS12 antibody 16455-1-APDownload protocol
IHC protocol for INTS12 antibody 16455-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

INTS13 variants causing a recessive developmental ciliopathy disrupt assembly of the Integrator complex

Authors - Lauren G. Mascibroda
View Article
humanWB

PLoS Genet

Human mutations in integrator complex subunits link transcriptome integrity to brain development.

Authors - Renske Oegema
View Article
humanWB

Cell Rep

A homozygous variant in INTS11 links mitosis and neurogenesis defects to a severe neurodevelopmental disorder

Authors - Hanzhe Kuang
View Article