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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated INTS4 Recombinant antibody

INTS4 Recombinant Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

240579H4

Cat no : CL488-83590-5

Synonyms

MSTP093, MST093, Int4, 240579H4

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated INTS4 Recombinant an (CL488-83590-5)

    IF Staining of HeLa using CL488-83590-5

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 INTS4 antibody (CL488-83590-5, Clone: 240579H4 ) at dilution of 1:200.

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Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL488-83590-5 is INTS4 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen INTS4 fusion protein Ag9116 相同性解析による交差性が予測される生物種
Full Name integrator complex subunit 4
Calculated molecular weight963aa,108 kDa; 505aa,57 kDa
GenBank accession numberBC009995
Gene symbol INTS4
Gene ID (NCBI) 92105
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Integrator complex subunit 4 (INTS4), a helical repeat protein, plays a key role in stabilizing nuclease domains and other components. INTS4 is a specific and conserved interaction partner of INTS9/11 heterodimer that does not interact with either subunit individually (PMID: 29471365). INTS4 plays a role in achieving a compact nuclease/pseudonuclease configuration resembling that observed for CPSF73/100 in the histone-processing machinery (PMID: 33548203).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 INTS4 antibody CL488-83590-5Download protocol
Standard Protocols
Click here to view our Standard Protocols