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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

INTS5 Recombinant antibody

INTS5 Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230462B10

Cat no : 83154-1-RR

Synonyms

INTS 5, Int5, 230462B10

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using INTS5 Recombinant antibody (83154-1-RR)

    WB analysis using 83154-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83154-1-RR (INTS5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using INTS5 Recombinant antibody (83154-1-RR)

    IF Staining of U2OS using 83154-1-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using INTS5 antibody (83154-1-RR, Clone: 230462B10 ) at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using INTS5 Recombinant antibody (83154-1-RR)

    FC experiment of HeLa using 83154-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug INTS5 Recombinant antibody (83154-1-RR, Clone:230462B10) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83154-1-RR

    Affinity and Kinetic Characterization of 83154-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83154-1-RR against Human INTS5 were performed. The affinity constant is 0.968 nM.

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Tested Applications

Positive WB detected inHeLa cells, NIH/3T3 cells, HCT-116 cells, HT-29 cells, SKOV-3 cells
Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83154-1-RR targets INTS5 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen INTS5 fusion protein Ag5129 相同性解析による交差性が予測される生物種
Full Name integrator complex subunit 5
Calculated molecular weight 108 kDa
Observed molecular weight108 kDa
GenBank accession numberBC060841
Gene symbol INTS5
Gene ID (NCBI) 80789
RRIDAB_3670848
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

INTS5, also named as INT5 and KIAA1698, is a component of the Integrator complex which involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes.

Protocols

Product Specific Protocols
WB protocol for INTS5 antibody 83154-1-RRDownload protocol
IF protocol for INTS5 antibody 83154-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols