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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using INTS6 Polyclonal antibody (31664-1-AP)

    WB analysis using 31664-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31664-1-AP (INTS6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using INTS6 Polyclonal antibody (31664-1-AP)

    WB analysis using 31664-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31664-1-AP (INTS6 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using INTS6 Polyclonal antibody (31664-1-AP)

    IHC staining of human ovary cancer using 31664-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 31664-1-AP (INTS6 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

INTS6 Polyclonal antibody

INTS6 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 31664-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

HDB, DICE1, DDX26A, DDX26, DBI1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inDU 145 cells, HuH-7 cells, L02 cells, PC-3 cells, LNCaP cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31664-1-AP targets INTS6 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen INTS6 fusion protein Ag36146 相同性解析による交差性が予測される生物種
Full Name integrator complex subunit 6
Calculated molecular weight 100 kDa
Observed molecular weight130 kDa
GenBank accession numberBC039829
Gene Symbol INTS6
Gene ID (NCBI) 26512
RRIDAB_3670067
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ9UL03
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

INTS6 is a component of the Integrator (INT) complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing.

Protocols

Product Specific Protocols
WB protocol for INTS6 antibody 31664-1-APDownload protocol
IHC protocol for INTS6 antibody 31664-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols