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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

INTS7 Polyclonal antibody, PBS Only

INTS7 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 17054-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using INTS7 Polyclonal antibody (17054-1-AP)

    WB analysis of HEK-293 using 17054-1-AP (same clone as 17054-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 17054-1-AP (INTS7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17054-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A431 cells using INTS7 Polyclonal antibody (17054-1-AP)

    WB analysis of A431 using 17054-1-AP (same clone as 17054-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 17054-1-AP (INTS7 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17054-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using INTS7 Polyclonal antibody (17054-1-AP)

    IHC staining of human brain using 17054-1-AP (same clone as 17054-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 17054-1-AP (INTS7 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 17054-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using INTS7 Polyclonal antibody (17054-1-AP)

    IHC staining of human brain using 17054-1-AP (same clone as 17054-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 17054-1-AP (INTS7 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 17054-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using INTS7 Polyclonal antibody (17054-1-AP)

    IF Staining of SH-SY5Y using 17054-1-AP (same clone as 17054-1-PBS)

    Immunofluorescent analysis of SH-SY5Y cells using 17054-1-AP (INTS7 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 17054-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

17054-1-PBS targets INTS7 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen INTS7 fusion protein Ag10755 相同性解析による交差性が予測される生物種
Full Name integrator complex subunit 7
Calculated molecular weight 948 aa, 105 kDa
Observed molecular weight 107 kDa
GenBank accession numberBC020523
Gene symbol INTS7
Gene ID (NCBI) 25896
RRIDAB_2127393
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

INTS7 is a subunit of the integrator complex. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes. Plays a role in DNA damage response (DDR) signaling during the S phase (PMID: 21659603).