INTS9 Polyclonal antibody

Name: INTS9 Polyclonal antibody
Brand: Proteintech
SKU: 11657-1-AP

INTS9 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 11657-1-AP

Synonyms

RC 74, INTS 9, Int9, CPSF2L, CPSF2

Validation Data Gallery

Filter:

AllWBIPIHC

WB analysis of Jurkat using 11657-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 11657-1-AP (INTS9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 11657-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 11657-1-AP (INTS9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 11657-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 11657-1-AP (INTS9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 11657-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 11657-1-AP (INTS9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 11657-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 11657-1-AP (INTS9 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 11657-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 11657-1-AP (INTS9 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 11657-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 11657-1-AP (INTS9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 11657-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 11657-1-AP (INTS9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 11657-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 11657-1-AP (INTS9 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 11657-1-AP
IP result of anti-INTS9 (IP:11657-1-AP, 4ug; Detection:11657-1-AP 1:1000) with HeLa cells lysate 880ug.
IHC staining of human stomach using 11657-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 11657-1-AP (INTS9 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Cat No. 11657-1-AP

Size/Concentration

ご購入について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	Jurkat cells, HEK-293 cells, HeLa cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human stomach tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 5 publications below
IP	See 2 publications below

Product Information

11657-1-AP targets INTS9 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	INTS9 fusion protein Ag2226 相同性解析による交差性が予測される生物種
Full Name	integrator complex subunit 9
Calculated molecular weight	658 aa, 74 kDa
Observed molecular weight	74 kDa
GenBank accession number	BC025267
Gene symbol	INTS9
Gene ID (NCBI)	55756
RRID	AB_2127514
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for INTS9 antibody 11657-1-AP	Download protocol
IHC protocol for INTS9 antibody 11657-1-AP	Download protocol
IP protocol for INTS9 antibody 11657-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Mol Cell Assembly mechanism of Integrator's RNA cleavage module Authors - Kevin Sabath View Article
human	WB	Nat Commun INTS10-INTS13-INTS14 form a functional module of Integrator that binds nucleic acids and the cleavage module. Authors - Kevin Sabath View Article
human	WB	Protein Expr Purif CRISPR-Cas9 mediated genetic engineering for the purification of the endogenous integrator complex from mammalian cells. Authors - David Baillat View Article
human	WB,IP	Mol Cell Integrator facilitates RNAPII removal to prevent transcription-replication collisions and genome instability Authors - Rahul Bhowmick View Article
human	WB,IP	Mol Cell Cytoplasmic binding partners of the Integrator endonuclease INTS11 and its paralog CPSF73 are required for their nuclear function Authors - Min-Han Lin View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

INTS9 Polyclonal antibody

INTS9 Polyclonal Antibody for WB, IHC, IP, ELISA

Validation Data Gallery

WB analysis of Jurkat using 11657-1-AP

WB analysis of HeLa using 11657-1-AP

WB analysis of HeLa using 11657-1-AP

WB analysis of HEK-293 using 11657-1-AP

WB analysis of Jurkat using 11657-1-AP

WB analysis of Jurkat using 11657-1-AP

WB analysis of HEK-293 using 11657-1-AP

WB analysis of Jurkat using 11657-1-AP

WB analysis of Jurkat using 11657-1-AP

IP experiment of HeLa using 11657-1-AP

IHC staining of human stomach using 11657-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Assembly mechanism of Integrator's RNA cleavage module

INTS10-INTS13-INTS14 form a functional module of Integrator that binds nucleic acids and the cleavage module.

CRISPR-Cas9 mediated genetic engineering for the purification of the endogenous integrator complex from mammalian cells.

Integrator facilitates RNAPII removal to prevent transcription-replication collisions and genome instability

Cytoplasmic binding partners of the Integrator endonuclease INTS11 and its paralog CPSF73 are required for their nuclear function