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- KD/KO Validated
INVS Polyclonal antibody
INVS Polyclonal Antibody for WB, IF, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IF, FC, IHC, ELISA
Conjugate
Unconjugated
Cat no : 10585-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | MCF-7 cells, K-562 cells, L02 cells, SH-SY5Y cells, mouse skeletal muscle tissue, HepG2 cells, Raji cells, Jurkat cells |
| Positive IHC detected in | human breast cancer tissue, human hepatocirrhosis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Hela cells, hTERT-RPE1 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 5 publications below |
| IHC | See 1 publications below |
| IF | See 4 publications below |
| FC | See 1 publications below |
Product Information
10585-1-AP targets INVS in WB, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, rat, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | INVS fusion protein Ag0934 相同性解析による交差性が予測される生物種 |
| Full Name | inversin |
| Calculated molecular weight | 118 kDa, 100 kDa |
| Observed molecular weight | 100-110 kDa |
| GenBank accession number | BC006370 |
| Gene symbol | INVS |
| Gene ID (NCBI) | 27130 |
| RRID | AB_2233902 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
INVS, short for inversin, plays a role in primary cilia function and involvement in the cell cycle. It is required for normal renal development and establishment of the left-right axis. INVS may be involved in Wnt signaling pathways by acting as a molecular switch between different them. Inhibits the canonical Wnt pathway by targeting cytoplasmic disheveled (DVL1) for degradation by the ubiquitin-proteasome. This suggests that it is required in renal development to oppose the repression of terminal differentiation of tubular epithelial cells by Wnt signaling. This antibody is a rabbit polyclonal antibody raised against part of the N-terminus of human INVS.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for INVS antibody 10585-1-AP | Download protocol |
| IHC protocol for INVS antibody 10585-1-AP | Download protocol |
| IF protocol for INVS antibody 10585-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
EMBO J Cell type-specific regulation of ciliary transition zone assembly in vertebrates.
| ||
Proc Natl Acad Sci U S A A complement factor H homolog, heparan sulfation, and syndecan maintain inversin compartment boundaries in C. elegans cilia. | ||
Endocrinology PCP protein inversin regulates testis function through changes in cytoskeletal organization of actin and microtubules.
| ||
Virchows Arch Afferent arteriolopathy and glomerular collapse but not segmental sclerosis induce tubular atrophy in old spontaneously hypertensive rats. | ||
Am J Med Genet A Early presentation of cystic kidneys in a family with a homozygous INVS mutation. | ||
