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Validation Data Gallery

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  • Western Blot (WB) analysis of human adrenal gland tissue using IP6K2 Polyclonal antibody (13155-1-AP)

    WB analysis of human adrenal gland using 13155-1-AP

    human adrenal gland tissue were subjected to SDS PAGE followed by western blot with 13155-1-AP (IP6K2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using IP6K2 Polyclonal antibody (13155-1-AP)

    WB analysis of human heart using 13155-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 13155-1-AP (IP6K2 antibody) at dilution of 1:1800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using IP6K2 Polyclonal antibody (13155-1-AP)

    WB analysis of K-562 using 13155-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 13155-1-AP (IP6K2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using IP6K2 Polyclonal antibody (13155-1-AP)

    IHC staining of human liver cancer using 13155-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13155-1-AP (IP6K2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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IP6K2 Polyclonal antibody

IP6K2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 13155-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

IHPK2, InsP6 kinase 2, IP6K2, P(i) uptake stimulator, PiUS

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman adrenal gland tissue, human heart tissue, K-562 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3600
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13155-1-AP targets IP6K2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IP6K2 fusion protein Ag3786 相同性解析による交差性が予測される生物種
Full Name inositol hexakisphosphate kinase 2
Calculated molecular weight 426 aa, 49 kDa
Observed molecular weight 49 kDa
GenBank accession numberBC019694
Gene Symbol IP6K2
Gene ID (NCBI) 51447
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UHH9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IP6K2(Inositol hexakisphosphate kinase 2) is also named as IHPK2, PiUS and belongs to the inositol phosphokinase (IPK) family. It converts inositol hexakisphosphate (InsP6) to diphosphoinositol pentakisphosphate (InsP7/PP-InsP5) and and mediates apoptotic cell death via its translocation from the nucleus to the cytoplasm(PMID:21652713). IP6K2 regulates TP53 and protein kinase AKT signaling, and its aberrant increase in cells has been implicated in apoptosis and INS resistance(PMID:21262846). It has 6 isoforms produced by alternative splicing. This antibody recognizes the others except the 8 kDa isoform of IP6K2.

Protocols

Product Specific Protocols
WB protocol for IP6K2 antibody 13155-1-APDownload protocol
IHC protocol for IP6K2 antibody 13155-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols