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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IPMK Polyclonal antibody (30161-1-AP)

    WB analysis using 30161-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30161-1-AP (IPMK antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using IPMK Polyclonal antibody (30161-1-AP)

    IF Staining of A431 using 30161-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using IPMK antibody (30161-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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IPMK Polyclonal antibody

IPMK Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 30161-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Synonyms

IMPK, IPMK

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA431 cells, HeLa cells, HepG2 cells, MCF-7 cells
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30161-1-AP targets IPMK in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IPMK fusion protein Ag32377 相同性解析による交差性が予測される生物種
Full Name inositol polyphosphate multikinase
Observed molecular weight42-47 kDa
GenBank accession numberBC065709
Gene Symbol IPMK
Gene ID (NCBI) 253430
RRIDAB_3086252
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NFU5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IPMK plays an important role in MLKL-mediated necroptosis via its role in the biosynthesis of inositol pentakisphosphate (InsP5) and inositol hexakisphosphate (InsP6). It is required for normal embryonic development, probably via its role in the biosynthesis of inositol 1,3,4,5,6-pentakisphosphate (Ins(1,3,4,5,6)P5) and inositol hexakisphosphate (InsP6).

Protocols

Product Specific Protocols
WB protocol for IPMK antibody 30161-1-APDownload protocol
IF protocol for IPMK antibody 30161-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols