Validation Data Gallery
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Tested Applications
| Positive WB detected in | MCF-7 cells, human brain tissue, Neura-2a cells, mouse lung |
| Positive IHC detected in | human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 5 publications below |
| IHC | See 1 publications below |
| IF | See 2 publications below |
| IP | See 1 publications below |
Product Information
11696-2-AP targets IPO13 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | IPO13 fusion protein Ag2317 相同性解析による交差性が予測される生物種 |
| Full Name | importin 13 |
| Calculated molecular weight | 963 aa, 108 kDa |
| Observed molecular weight | 97 kDa |
| GenBank accession number | BC008194 |
| Gene Symbol | IPO13 |
| Gene ID (NCBI) | 9670 |
| RRID | AB_2127672 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O94829 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for IPO13 antibody 11696-2-AP | Download protocol |
| IHC protocol for IPO13 antibody 11696-2-AP | Download protocol |
| IF protocol for IPO13 antibody 11696-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Nuclear transporter Importin-13 plays a key role in the oxidative stress transcriptional response.
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Stem Cells Importin 13 serves as a potential marker for corneal epithelial progenitor cells. | ||
Data Brief Transcriptomic profile dataset of embryonic stem cells (Wild-type and IPO13-Knock Out) with and without oxidative stress.
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Cells The Nuclear Transporter Importin 13 Can Regulate Stress-Induced Cell Death through the Clusterin/KU70 Axis
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