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KD/KO Validated

IPO13 Polyclonal antibody

Name: IPO13 Polyclonal antibody
Brand: Proteintech
SKU: 11696-2-AP

IPO13 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 11696-2-AP

Synonyms

KIAA0724, Karyopherin-13, Karyopherin 13, Kap13, Importin-13

Validation Data Gallery

Filter:

AllWBIHCIF/ICC

WB analysis using 11696-2-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11696-2-AP (IPO13 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 11696-2-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 11696-2-AP (IPO13 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human gliomas using 11696-2-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11696-2-AP (IPO13 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa cells using 11696-2-AP
IF result in Hela cell from Dr. Ralph Kehlenbach showed Nuclear and Cytoplasma stain.
IF Staining of HeLa using 11696-2-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using IPO13 antibody (11696-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Cat No. 11696-2-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	MCF-7 cells, human brain tissue, Neura-2a cells, mouse lung
Positive IHC detected in	human gliomas tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 4 publications below
IHC	See 1 publications below
IF	See 2 publications below
IP	See 1 publications below

Product Information

11696-2-AP targets IPO13 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	IPO13 fusion protein Ag2317 相同性解析による交差性が予測される生物種
Full Name	importin 13
Calculated molecular weight	963 aa, 108 kDa
Observed molecular weight	97 kDa
GenBank accession number	BC008194
Gene symbol	IPO13
Gene ID (NCBI)	9670
RRID	AB_2127672
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for IPO13 antibody 11696-2-AP	Download protocol
IHC protocol for IPO13 antibody 11696-2-AP	Download protocol
IF protocol for IPO13 antibody 11696-2-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IF,IP	Nat Commun Nuclear transporter Importin-13 plays a key role in the oxidative stress transcriptional response. Authors - K A Gajewska KD Validated View Article
human	WB,IHC,IF	Stem Cells Importin 13 serves as a potential marker for corneal epithelial progenitor cells. Authors - Wang Hua H View Article
mouse	WB	Data Brief Transcriptomic profile dataset of embryonic stem cells (Wild-type and IPO13-Knock Out) with and without oxidative stress. Authors - Katarzyna A Gajewska KO Validated View Article
human	WB	Cells The Nuclear Transporter Importin 13 Can Regulate Stress-Induced Cell Death through the Clusterin/KU70 Axis Authors - Katarzyna A Gajewska KD Validated View Article

製品

アプリケーション

cGMPグレード製品

サポート

カスタム対応

プロモーション

企業情報

抗体

免疫アッセイ

サイトカイン・増殖因子

ChromoTek VHH（Nanobody®）試薬

サポートツール・試薬

ゲノミクス試薬

研究分野

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IPO13 Polyclonal antibody

IPO13 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Validation Data Gallery

WB analysis using 11696-2-AP

WB analysis of human brain using 11696-2-AP

IHC staining of human gliomas using 11696-2-AP

IF Staining of HeLa cells using 11696-2-AP

IF Staining of HeLa using 11696-2-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Nuclear transporter Importin-13 plays a key role in the oxidative stress transcriptional response.

Importin 13 serves as a potential marker for corneal epithelial progenitor cells.

Transcriptomic profile dataset of embryonic stem cells (Wild-type and IPO13-Knock Out) with and without oxidative stress.

The Nuclear Transporter Importin 13 Can Regulate Stress-Induced Cell Death through the Clusterin/KU70 Axis