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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IPO5 Monoclonal antibody (67962-1-Ig)

    WB analysis using 67962-1-Ig (same clone as 67962-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67962-1-Ig (IPO5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Lamin B1 Monoclonal antibody (66095-1-Ig) as loading control. This data was developed using the same antibody clone with 67962-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using IPO5 Monoclonal antibody (67962-1-Ig)

    IHC staining of mouse testis using 67962-1-Ig (same clone as 67962-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67962-1-Ig (IPO5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67962-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using IPO5 Monoclonal antibody (67962-1-Ig)

    IF Staining of A431 using 67962-1-Ig (same clone as 67962-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using IPO5 antibody (67962-1-Ig, Clone: 1E2B9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67962-1-PBS in a different storage buffer formulation.

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IPO5 Monoclonal antibody, PBS Only

IPO5 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67962-1-PBS
Clone No.1E2B9

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

DKFZp686O1576, FLJ43041, IMB3, Imp5, importin 5, Importin subunit beta 3, IPO5, Karyopherin beta 3, KPNB3, Ran binding protein 5, RANBP5

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67962-1-PBS targets IPO5 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen IPO5 fusion protein Ag30955 相同性解析による交差性が予測される生物種
Full Name importin 5
Observed molecular weight124 kDa
GenBank accession numberBC001497
Gene Symbol IPO5
Gene ID (NCBI) 3843
RRIDAB_2918713
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO00410
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

IPO5 is a member of the importin beta family. Structurally, the protein adopts the shape of a right-hand solenoid and is composed of 24 HEAT repeats. IPO5 facilitates cytoplasmic polyadenylation element-binding protein CPEB3 translocation by binding to the RRM1 motif of CPEB3 in neurons. NMDAR signaling increases RanBP1 expression and reduces the level of cytoplasmic GTP-bound Ran. These changes enhance CPEB3-IPO5 interaction, which consequently accelerates the nuclear import of CPEB3 and promotes its nuclear function.